Distribution of O-acetylated sialic acids among target host tissues for influenza virus

Brian R. Wasik, Karen N. Barnard, Robert J. Ossiboff, Zahra Khedri, Kurtis H. Feng, Hai Yu, Xi Chen, Daniel R. Perez, Ajit Varki, Colin R. Parrish

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Sialic acids (Sias) are important glycans displayed on the cells and tissues of many different animals and are frequent targets for binding and modification by pathogens, including influenza viruses. Influenza virus hemagglutinins bind Sias during the infection of their normal hosts, while the encoded neuraminidases and/or esterases remove or modify the Sia to allow virion release or to prevent rebinding. Sias naturally occur in a variety of modified forms, and modified Sias can alter influenza virus host tropisms through their altered interactions with the viral glycoproteins. However, the distribution of modified Sia forms and their effects on pathogen-host interactions are still poorly understood. Here we used probes developed from viral Sia-binding proteins to detect O-acetylated (4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl) Sias displayed on the tissues of some natural or experimental hosts for influenza viruses. These modified Sias showed highly variable displays between the hosts and tissues examined. The 9-O-acetyl (and 7,9-) modified Sia forms were found on cells and tissues of many hosts, including mice, humans, ferrets, guinea pigs, pigs, horses, dogs, as well as in those of ducks and embryonated chicken egg tissues and membranes, although in variable amounts. The 4-O-acetyl Sias were found in the respiratory tissues of fewer animals, being primarily displayed in the horse and guinea pig, but were not detected in humans or pigs. The results suggest that these Sia variants may influence virus tropisms by altering and selecting their cell interactions.

Original languageEnglish (US)
Article numbere00379-16
JournalmSphere
Volume2
Issue number5
DOIs
StatePublished - Sep 1 2017

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Sialic Acids
Orthomyxoviridae
Horses
Guinea Pigs
Swine
Viral Tropism
Host-Pathogen Interactions
Ferrets
Tropism
Ducks
Hemagglutinins
Neuraminidase
Esterases
Cell Communication
Virion
Polysaccharides
Ovum
Chickens
Glycoproteins
Carrier Proteins

Keywords

  • Host range
  • Influenza
  • Receptor-ligand interaction
  • Respiratory viruses
  • Sialic acid
  • Virus-host interactions

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Wasik, B. R., Barnard, K. N., Ossiboff, R. J., Khedri, Z., Feng, K. H., Yu, H., ... Parrish, C. R. (2017). Distribution of O-acetylated sialic acids among target host tissues for influenza virus. mSphere, 2(5), [e00379-16]. https://doi.org/10.1128/mSphere.00379-16

Distribution of O-acetylated sialic acids among target host tissues for influenza virus. / Wasik, Brian R.; Barnard, Karen N.; Ossiboff, Robert J.; Khedri, Zahra; Feng, Kurtis H.; Yu, Hai; Chen, Xi; Perez, Daniel R.; Varki, Ajit; Parrish, Colin R.

In: mSphere, Vol. 2, No. 5, e00379-16, 01.09.2017.

Research output: Contribution to journalArticle

Wasik, BR, Barnard, KN, Ossiboff, RJ, Khedri, Z, Feng, KH, Yu, H, Chen, X, Perez, DR, Varki, A & Parrish, CR 2017, 'Distribution of O-acetylated sialic acids among target host tissues for influenza virus', mSphere, vol. 2, no. 5, e00379-16. https://doi.org/10.1128/mSphere.00379-16
Wasik BR, Barnard KN, Ossiboff RJ, Khedri Z, Feng KH, Yu H et al. Distribution of O-acetylated sialic acids among target host tissues for influenza virus. mSphere. 2017 Sep 1;2(5). e00379-16. https://doi.org/10.1128/mSphere.00379-16
Wasik, Brian R. ; Barnard, Karen N. ; Ossiboff, Robert J. ; Khedri, Zahra ; Feng, Kurtis H. ; Yu, Hai ; Chen, Xi ; Perez, Daniel R. ; Varki, Ajit ; Parrish, Colin R. / Distribution of O-acetylated sialic acids among target host tissues for influenza virus. In: mSphere. 2017 ; Vol. 2, No. 5.
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