Distribution of MHC class II antigens in feline tissues and peripheral blood

Bruce A. Rideout, Peter F Moore, Niels C Pedersen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Abstract: A new monoclonal antibody raised against gradient‐purified feline immunodeficiency virus was found to recognize a bimolecular complex, comprising 27–29 kD and 32–35 kD subunits, on feline peripheral blood lymphocytes. Immunoperoxidase staining of feline tissues with this antibody, designated 43.2H2, demonstrated a reactivity pattern similar to that described for MHC II antigens of the dog, horse, and pig, but differed from human and mouse in having staining of T‐cell zones in spleen and lymph nodes. Flow cytometric analysis revealed that 42.3H2 reacted with 88.97%± 16.00% of feline peripheral blood lymphocytes (n = 20). This high level of reactivity was found to be consistent by repeated sampling over a 4‐month period. Two‐color flow cytometric analysis was used to determined the reactivity pattern on lymphocyte subsets: 88.92%± 7.30% of CD4+ lymphocytes were 42.3H2‐positive, while 85.99%± 11.46% of CD8+ cells were positive (n = 11 for both). B lymphocytes had the highest reactivity (99.47%± 0.45; n=9) and also had the highest fluorescence intensity. By gating based on light scatter properties, 95.06%± 7.35% of monocytes were 42.3H2‐reactive (n= 18), while granulocytes were negative.

Original languageEnglish (US)
Pages (from-to)221-227
Number of pages7
JournalTissue Antigens
Volume36
Issue number5
DOIs
StatePublished - 1990

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Histocompatibility Antigens Class II
Felidae
Lymphocytes
Staining and Labeling
Feline Immunodeficiency Virus
Lymphocyte Subsets
Granulocytes
Horses
Monocytes
B-Lymphocytes
Swine
Spleen
Fluorescence
Lymph Nodes
Monoclonal Antibodies
Dogs
Light
Antigens
Antibodies

Keywords

  • feline
  • FIV
  • flow cytometry
  • immunoperoxidase
  • lymphocyte
  • MHC
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Genetics

Cite this

Distribution of MHC class II antigens in feline tissues and peripheral blood. / Rideout, Bruce A.; Moore, Peter F; Pedersen, Niels C.

In: Tissue Antigens, Vol. 36, No. 5, 1990, p. 221-227.

Research output: Contribution to journalArticle

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