Distribution of ions around DNA, probed by energy transfer

T. G. Wensel, C. F. Meares, V. Vlachy, J. B. Matthew

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Measurements of the effect of DNA on rates of bimolecular energy transfer between ions provide a direct indication of how cations cluster in regions near DNA and how anions are repelled from the same regions. Energy transfer from luminescent lanthanide ions (in the 'rapid-diffusion' limit) probes collision frequencies that are dependent on the equilibrium spatial distributions of ions. The addition of 1 mM DNA (phosphate) to a 2 mM salt solution increases the overall collision frequency between monovalent cations by a factor of 6 ± 1.5; it increases the divalent-monovalent cation collision frequency by a factor of 29 ± 3; and it decreases the divalent cation-monovalent anion collision frequency by a factor of 0.24 ± 0.03. Comparisons are made with the changes in collision frequencies predicted by several different theoretical descriptions of ion distributions. The closest agreement with experimental results for monovalent ions at 1 mM DNA is obtained with a static accessibility-modified discrete charge calculation, based on a detailed molecular model of B-DNA. At high DNA concentration (10 mM), the best results are obtained by numerical solutions of the Poisson-Boltzmann equation for a 'soft-rod' model of DNA. Poisson-Boltzmann calculations for a 'hard-rod' model greatly overestimate the effects of DNA on collision frequencies, as does a calculation based on counterion-condensation theory.

Original languageEnglish (US)
Pages (from-to)3267-3271
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number10
StatePublished - 1986

ASJC Scopus subject areas

  • General
  • Genetics

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