Distribution of introns in frameshift-suppressor proline-tRNA genes of Saccharomyces cerevisiae

Mark Winey, Lorilee Mathison, Cheryl M. Soref, Michael R. Culbertson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mutations in the suf9, suf10, and suf11 genes of yeast suppress + 1 nucleotide (nt) insertions in proline codons. Nucleotide sequence analysis indicates that the suf9 and suf11 genes are members of the proline tRNAUGGgene family, which also includes three other previously identified genes, suf7, suf8, and trn1. All five members of this gene family contain introns. The suf9 and suf11 introns are 31 and 30 nt in length, respectively, and are similar but not identical in sequence to other introns within the family. The suf10 gene is identical in sequence to suf2, which was shown previously to encode proline tRNAIGG. Both members of this gene family lack introns. Alleles of suf9, suf10, and suf11 that confer frameshift suppression were also analyzed. The SUF9-1. allele results in a G→U substitution at nt position 39 in the anticodon stem. The recessive suf 11-1 allele is a double mutant containing the same nt position 39 alteration as in SUF9-1 plus a second U→A substitution at nt position 38 in the anticodon loop. The SUF10-1 suppressor mutation corresponds to a + 1G insertion in the anticodon loop. Since the nt substitutions in suf11-1 alter the sequence of the 3′ exon/intron boundary, the double mutant pre-tRNA was tested for its ability to be cleaved in vitro by tRNA-splicing endonuclease. It was found that suf11-1 pre-tRNA is cleaved with reduced efficiency at the 3′ splice junction.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalGene
Volume76
Issue number1
DOIs
StatePublished - Mar 15 1989
Externally publishedYes

Fingerprint

Transfer RNA
Proline
Introns
Saccharomyces cerevisiae
Nucleotides
Anticodon
Genes
Alleles
RNA Precursors
Genetic Suppression
Aptitude
Codon
Sequence Analysis
Exons
Yeasts
Efficiency
Mutation

Keywords

  • anticodon
  • Base substitution
  • codon
  • introns
  • recombinant DNA
  • translation
  • tRNA splicing
  • yeast

ASJC Scopus subject areas

  • Genetics

Cite this

Distribution of introns in frameshift-suppressor proline-tRNA genes of Saccharomyces cerevisiae. / Winey, Mark; Mathison, Lorilee; Soref, Cheryl M.; Culbertson, Michael R.

In: Gene, Vol. 76, No. 1, 15.03.1989, p. 89-97.

Research output: Contribution to journalArticle

Winey, Mark ; Mathison, Lorilee ; Soref, Cheryl M. ; Culbertson, Michael R. / Distribution of introns in frameshift-suppressor proline-tRNA genes of Saccharomyces cerevisiae. In: Gene. 1989 ; Vol. 76, No. 1. pp. 89-97.
@article{bb1636e3c5244fb9a7e07a66d96ec5e0,
title = "Distribution of introns in frameshift-suppressor proline-tRNA genes of Saccharomyces cerevisiae",
abstract = "Mutations in the suf9, suf10, and suf11 genes of yeast suppress + 1 nucleotide (nt) insertions in proline codons. Nucleotide sequence analysis indicates that the suf9 and suf11 genes are members of the proline tRNAUGGgene family, which also includes three other previously identified genes, suf7, suf8, and trn1. All five members of this gene family contain introns. The suf9 and suf11 introns are 31 and 30 nt in length, respectively, and are similar but not identical in sequence to other introns within the family. The suf10 gene is identical in sequence to suf2, which was shown previously to encode proline tRNAIGG. Both members of this gene family lack introns. Alleles of suf9, suf10, and suf11 that confer frameshift suppression were also analyzed. The SUF9-1. allele results in a G→U substitution at nt position 39 in the anticodon stem. The recessive suf 11-1 allele is a double mutant containing the same nt position 39 alteration as in SUF9-1 plus a second U→A substitution at nt position 38 in the anticodon loop. The SUF10-1 suppressor mutation corresponds to a + 1G insertion in the anticodon loop. Since the nt substitutions in suf11-1 alter the sequence of the 3′ exon/intron boundary, the double mutant pre-tRNA was tested for its ability to be cleaved in vitro by tRNA-splicing endonuclease. It was found that suf11-1 pre-tRNA is cleaved with reduced efficiency at the 3′ splice junction.",
keywords = "anticodon, Base substitution, codon, introns, recombinant DNA, translation, tRNA splicing, yeast",
author = "Mark Winey and Lorilee Mathison and Soref, {Cheryl M.} and Culbertson, {Michael R.}",
year = "1989",
month = "3",
day = "15",
doi = "10.1016/0378-1119(89)90011-5",
language = "English (US)",
volume = "76",
pages = "89--97",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Distribution of introns in frameshift-suppressor proline-tRNA genes of Saccharomyces cerevisiae

AU - Winey, Mark

AU - Mathison, Lorilee

AU - Soref, Cheryl M.

AU - Culbertson, Michael R.

PY - 1989/3/15

Y1 - 1989/3/15

N2 - Mutations in the suf9, suf10, and suf11 genes of yeast suppress + 1 nucleotide (nt) insertions in proline codons. Nucleotide sequence analysis indicates that the suf9 and suf11 genes are members of the proline tRNAUGGgene family, which also includes three other previously identified genes, suf7, suf8, and trn1. All five members of this gene family contain introns. The suf9 and suf11 introns are 31 and 30 nt in length, respectively, and are similar but not identical in sequence to other introns within the family. The suf10 gene is identical in sequence to suf2, which was shown previously to encode proline tRNAIGG. Both members of this gene family lack introns. Alleles of suf9, suf10, and suf11 that confer frameshift suppression were also analyzed. The SUF9-1. allele results in a G→U substitution at nt position 39 in the anticodon stem. The recessive suf 11-1 allele is a double mutant containing the same nt position 39 alteration as in SUF9-1 plus a second U→A substitution at nt position 38 in the anticodon loop. The SUF10-1 suppressor mutation corresponds to a + 1G insertion in the anticodon loop. Since the nt substitutions in suf11-1 alter the sequence of the 3′ exon/intron boundary, the double mutant pre-tRNA was tested for its ability to be cleaved in vitro by tRNA-splicing endonuclease. It was found that suf11-1 pre-tRNA is cleaved with reduced efficiency at the 3′ splice junction.

AB - Mutations in the suf9, suf10, and suf11 genes of yeast suppress + 1 nucleotide (nt) insertions in proline codons. Nucleotide sequence analysis indicates that the suf9 and suf11 genes are members of the proline tRNAUGGgene family, which also includes three other previously identified genes, suf7, suf8, and trn1. All five members of this gene family contain introns. The suf9 and suf11 introns are 31 and 30 nt in length, respectively, and are similar but not identical in sequence to other introns within the family. The suf10 gene is identical in sequence to suf2, which was shown previously to encode proline tRNAIGG. Both members of this gene family lack introns. Alleles of suf9, suf10, and suf11 that confer frameshift suppression were also analyzed. The SUF9-1. allele results in a G→U substitution at nt position 39 in the anticodon stem. The recessive suf 11-1 allele is a double mutant containing the same nt position 39 alteration as in SUF9-1 plus a second U→A substitution at nt position 38 in the anticodon loop. The SUF10-1 suppressor mutation corresponds to a + 1G insertion in the anticodon loop. Since the nt substitutions in suf11-1 alter the sequence of the 3′ exon/intron boundary, the double mutant pre-tRNA was tested for its ability to be cleaved in vitro by tRNA-splicing endonuclease. It was found that suf11-1 pre-tRNA is cleaved with reduced efficiency at the 3′ splice junction.

KW - anticodon

KW - Base substitution

KW - codon

KW - introns

KW - recombinant DNA

KW - translation

KW - tRNA splicing

KW - yeast

UR - http://www.scopus.com/inward/record.url?scp=0024594364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024594364&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(89)90011-5

DO - 10.1016/0378-1119(89)90011-5

M3 - Article

C2 - 2663651

AN - SCOPUS:0024594364

VL - 76

SP - 89

EP - 97

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -