Distribution of FGF-5 in the rhesus macaque retina

T. Kitaoka, A. E. Aotaki-Keen, Leonard M Hjelmeland

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Purpose. To determine the distribution of mRNA transcribed from the FGF-5 gene and the distribution of FGF-5 protein in the normal adult retina of the rhesus macaque. Methods. Freshly enucleated globes from rhesus macaque were prepared for Northern blot analysis, in situ hybridization, and immunohistochemical studies. A 350 base pair sequence from the human FGF-5 was subcloned and used to prepare 35S-labeled cRNAs for Northern and in situ experiments. Antipeptide antibodies were raised against an aminoterminal sequence as well as an internal sequence and were affinity purified on recombinant FGF-5 coupled to AffiGel-10. The specificity of antibody probes was verified by dot blot and Western blot analyses. Results. Ganglion cells and photoreceptors express the highest levels of FGF-5 mRNA, although all neurons and the retinal pigment epithelium (RPE) were clearly labeled. Immunohistochemical staining similarly revealed a widespread distribution of the protein, with prominent labeling observed in photoreceptor inner segments, especially in cone cells. Plexiform layers and RPE cells were also clearly labeled. Conclusions. Clear evidence was established for the expression of FGF-5 in the retina, where it may play an important extracellular role as a secreted member of the FGF gene family.

Original languageEnglish (US)
Pages (from-to)3189-3198
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume35
Issue number8
StatePublished - 1994

Fingerprint

Retinal Pigment Epithelium
Macaca mulatta
Retina
Complementary RNA
Photoreceptor Cells
Messenger RNA
Antibody Specificity
Base Pairing
Ganglia
Northern Blotting
Genes
In Situ Hybridization
Proteins
Western Blotting
Staining and Labeling
Neurons
Antibodies

Keywords

  • FGF-5
  • immunohistochemistry
  • in situ hybridization
  • rhesus macaque retina

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Distribution of FGF-5 in the rhesus macaque retina. / Kitaoka, T.; Aotaki-Keen, A. E.; Hjelmeland, Leonard M.

In: Investigative Ophthalmology and Visual Science, Vol. 35, No. 8, 1994, p. 3189-3198.

Research output: Contribution to journalArticle

Kitaoka, T. ; Aotaki-Keen, A. E. ; Hjelmeland, Leonard M. / Distribution of FGF-5 in the rhesus macaque retina. In: Investigative Ophthalmology and Visual Science. 1994 ; Vol. 35, No. 8. pp. 3189-3198.
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