Distribution of dengue 2 antigens by electron immunocytochemistry

Robert Cardiff, J. K. Lund

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The distribution of dengue 2 antigens was studied in infected monkey kidney cells (LLC MK2) using an indirect, horseradish peroxidase conjugated immunoglobulin technique. This procedure allowed both light and electron microscopic examination of serial step sections of individual cells cut in a plane perpendicular to the monolayer. Both virion and nonvirion antigens were identified on the plasma membrane with this technique. A diffuse cytoplasmic reaction product was also present. The intensity and distribution of the cytoplasmic reaction product was related to disruption of the plasma membrane.

Original languageEnglish (US)
Pages (from-to)1699-1709
Number of pages11
JournalInfection and Immunity
Volume13
Issue number6
StatePublished - Dec 1 1976

Fingerprint

Dengue
Immunohistochemistry
Cell Membrane
Electrons
Antigens
Horseradish Peroxidase
Virion
Haplorhini
Immunoglobulins
Kidney
Light

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Distribution of dengue 2 antigens by electron immunocytochemistry. / Cardiff, Robert; Lund, J. K.

In: Infection and Immunity, Vol. 13, No. 6, 01.12.1976, p. 1699-1709.

Research output: Contribution to journalArticle

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