Distribution of cytochrome P‐450 monoxygenase enzymes in the nasal mucosa of hamster and rat

Donald R. Adams, Alisa M. Jones, Charles Plopper, Cosette J. Serabjit‐Singh, Richard M. Philpot

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Deposition of inhaled particulates onto the respiratory mucosa is relatively great in that portion of the nasal cavity unprotected by ciliated, goblet, or keratinized superficial cells. The cytochrome P‐450 system is an important enzyme system involved in the biotransformation of xenobiotics into metabolites that are more readily absorbed. To examine the transitional region caudal to the nasal vestibule, nasal tissues of hamster and rat were prepared for immunocytochemistry. Blocks of tissue representing four levels along the axis of the nasal cavity were examined. Paraffin sections were processed through the avidin‐biotin peroxidase procedure, with diaminobenzidine tetrahydrochloride as the chromagen. Enzyme localization was accomplished through the use of antibodies for three rabbit cytochrome P‐450 isozymes; 2, 5, and 6 (subfamilies IIB, IVB, and IA, respectively); and for rabbit NADPH‐cytochrome P‐450 reductase. Enzyme distribution was similar in both hamster and rat nasal tissues except in cells of striated and intercalated ducts of nasal glands and in cells of the nasolacrimal duct where immunoreactivity for reductase and isozyme 2 was intense in nonciliated cells lining the nonolfactory epithelium, in sustentacular cells of the olfactory epithelium, and acinar cells of olfactory glands. Distribution of reaction products to isozyme 5 and 6 were similar to but not so intense as those of reductase an isozyme 2. Reaction products for reductase and isozyme 2 occurred generally in the same cellular and intracellular regions with the following exceptions: isozyme 2 was more concentrated in cells of striated ducts and of the nasolacrimal duct, and reductase was more abundant in intercalated ducts of nasal glands. Ciliated and goblet cells in epithelia lining much of the nasal cavity evidenced little reactivity; those ciliated cells adjacent to olfactory mucosa contained reaction product. It is concluded that the nonciliated epithelium adjacent to the internal ostium of the nose contains enzymes essential for biotransforming extrinsic particles that impinge on it from inhaled air currents.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalAmerican Journal of Anatomy
Volume190
Issue number3
DOIs
StatePublished - Jan 1 1991

Fingerprint

Nasal Mucosa
Cricetinae
Cytochrome P-450 Enzyme System
Nose
Isoenzymes
Oxidoreductases
Nasal Cavity
Nasolacrimal Duct
Olfactory Mucosa
Epithelium
Enzymes
Rabbits
Respiratory Mucosa
Goblet Cells
Acinar Cells
Xenobiotics
Biotransformation
Paraffin
Peroxidase
Immunohistochemistry

ASJC Scopus subject areas

  • Anatomy

Cite this

Distribution of cytochrome P‐450 monoxygenase enzymes in the nasal mucosa of hamster and rat. / Adams, Donald R.; Jones, Alisa M.; Plopper, Charles; Serabjit‐Singh, Cosette J.; Philpot, Richard M.

In: American Journal of Anatomy, Vol. 190, No. 3, 01.01.1991, p. 291-298.

Research output: Contribution to journalArticle

Adams, Donald R. ; Jones, Alisa M. ; Plopper, Charles ; Serabjit‐Singh, Cosette J. ; Philpot, Richard M. / Distribution of cytochrome P‐450 monoxygenase enzymes in the nasal mucosa of hamster and rat. In: American Journal of Anatomy. 1991 ; Vol. 190, No. 3. pp. 291-298.
@article{57ff6d87ba334bb28ab6b6c9b050899a,
title = "Distribution of cytochrome P‐450 monoxygenase enzymes in the nasal mucosa of hamster and rat",
abstract = "Deposition of inhaled particulates onto the respiratory mucosa is relatively great in that portion of the nasal cavity unprotected by ciliated, goblet, or keratinized superficial cells. The cytochrome P‐450 system is an important enzyme system involved in the biotransformation of xenobiotics into metabolites that are more readily absorbed. To examine the transitional region caudal to the nasal vestibule, nasal tissues of hamster and rat were prepared for immunocytochemistry. Blocks of tissue representing four levels along the axis of the nasal cavity were examined. Paraffin sections were processed through the avidin‐biotin peroxidase procedure, with diaminobenzidine tetrahydrochloride as the chromagen. Enzyme localization was accomplished through the use of antibodies for three rabbit cytochrome P‐450 isozymes; 2, 5, and 6 (subfamilies IIB, IVB, and IA, respectively); and for rabbit NADPH‐cytochrome P‐450 reductase. Enzyme distribution was similar in both hamster and rat nasal tissues except in cells of striated and intercalated ducts of nasal glands and in cells of the nasolacrimal duct where immunoreactivity for reductase and isozyme 2 was intense in nonciliated cells lining the nonolfactory epithelium, in sustentacular cells of the olfactory epithelium, and acinar cells of olfactory glands. Distribution of reaction products to isozyme 5 and 6 were similar to but not so intense as those of reductase an isozyme 2. Reaction products for reductase and isozyme 2 occurred generally in the same cellular and intracellular regions with the following exceptions: isozyme 2 was more concentrated in cells of striated ducts and of the nasolacrimal duct, and reductase was more abundant in intercalated ducts of nasal glands. Ciliated and goblet cells in epithelia lining much of the nasal cavity evidenced little reactivity; those ciliated cells adjacent to olfactory mucosa contained reaction product. It is concluded that the nonciliated epithelium adjacent to the internal ostium of the nose contains enzymes essential for biotransforming extrinsic particles that impinge on it from inhaled air currents.",
author = "Adams, {Donald R.} and Jones, {Alisa M.} and Charles Plopper and Serabjit‐Singh, {Cosette J.} and Philpot, {Richard M.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1002/aja.1001900309",
language = "English (US)",
volume = "190",
pages = "291--298",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Distribution of cytochrome P‐450 monoxygenase enzymes in the nasal mucosa of hamster and rat

AU - Adams, Donald R.

AU - Jones, Alisa M.

AU - Plopper, Charles

AU - Serabjit‐Singh, Cosette J.

AU - Philpot, Richard M.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Deposition of inhaled particulates onto the respiratory mucosa is relatively great in that portion of the nasal cavity unprotected by ciliated, goblet, or keratinized superficial cells. The cytochrome P‐450 system is an important enzyme system involved in the biotransformation of xenobiotics into metabolites that are more readily absorbed. To examine the transitional region caudal to the nasal vestibule, nasal tissues of hamster and rat were prepared for immunocytochemistry. Blocks of tissue representing four levels along the axis of the nasal cavity were examined. Paraffin sections were processed through the avidin‐biotin peroxidase procedure, with diaminobenzidine tetrahydrochloride as the chromagen. Enzyme localization was accomplished through the use of antibodies for three rabbit cytochrome P‐450 isozymes; 2, 5, and 6 (subfamilies IIB, IVB, and IA, respectively); and for rabbit NADPH‐cytochrome P‐450 reductase. Enzyme distribution was similar in both hamster and rat nasal tissues except in cells of striated and intercalated ducts of nasal glands and in cells of the nasolacrimal duct where immunoreactivity for reductase and isozyme 2 was intense in nonciliated cells lining the nonolfactory epithelium, in sustentacular cells of the olfactory epithelium, and acinar cells of olfactory glands. Distribution of reaction products to isozyme 5 and 6 were similar to but not so intense as those of reductase an isozyme 2. Reaction products for reductase and isozyme 2 occurred generally in the same cellular and intracellular regions with the following exceptions: isozyme 2 was more concentrated in cells of striated ducts and of the nasolacrimal duct, and reductase was more abundant in intercalated ducts of nasal glands. Ciliated and goblet cells in epithelia lining much of the nasal cavity evidenced little reactivity; those ciliated cells adjacent to olfactory mucosa contained reaction product. It is concluded that the nonciliated epithelium adjacent to the internal ostium of the nose contains enzymes essential for biotransforming extrinsic particles that impinge on it from inhaled air currents.

AB - Deposition of inhaled particulates onto the respiratory mucosa is relatively great in that portion of the nasal cavity unprotected by ciliated, goblet, or keratinized superficial cells. The cytochrome P‐450 system is an important enzyme system involved in the biotransformation of xenobiotics into metabolites that are more readily absorbed. To examine the transitional region caudal to the nasal vestibule, nasal tissues of hamster and rat were prepared for immunocytochemistry. Blocks of tissue representing four levels along the axis of the nasal cavity were examined. Paraffin sections were processed through the avidin‐biotin peroxidase procedure, with diaminobenzidine tetrahydrochloride as the chromagen. Enzyme localization was accomplished through the use of antibodies for three rabbit cytochrome P‐450 isozymes; 2, 5, and 6 (subfamilies IIB, IVB, and IA, respectively); and for rabbit NADPH‐cytochrome P‐450 reductase. Enzyme distribution was similar in both hamster and rat nasal tissues except in cells of striated and intercalated ducts of nasal glands and in cells of the nasolacrimal duct where immunoreactivity for reductase and isozyme 2 was intense in nonciliated cells lining the nonolfactory epithelium, in sustentacular cells of the olfactory epithelium, and acinar cells of olfactory glands. Distribution of reaction products to isozyme 5 and 6 were similar to but not so intense as those of reductase an isozyme 2. Reaction products for reductase and isozyme 2 occurred generally in the same cellular and intracellular regions with the following exceptions: isozyme 2 was more concentrated in cells of striated ducts and of the nasolacrimal duct, and reductase was more abundant in intercalated ducts of nasal glands. Ciliated and goblet cells in epithelia lining much of the nasal cavity evidenced little reactivity; those ciliated cells adjacent to olfactory mucosa contained reaction product. It is concluded that the nonciliated epithelium adjacent to the internal ostium of the nose contains enzymes essential for biotransforming extrinsic particles that impinge on it from inhaled air currents.

UR - http://www.scopus.com/inward/record.url?scp=0025964253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025964253&partnerID=8YFLogxK

U2 - 10.1002/aja.1001900309

DO - 10.1002/aja.1001900309

M3 - Article

C2 - 2048556

AN - SCOPUS:0025964253

VL - 190

SP - 291

EP - 298

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 3

ER -