Distribution of cytochrome P450 1A1 and NADPH-cytochrome P450 reductase in lungs of rabbits treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin

Ultrastructural immunolocalization and in situ hybridization

L. H. Overby, S. Nishio, A. Weir, G. T. Carver, Charles Plopper, R. M. Philpot

Research output: Contribution to journalArticle

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Abstract

Induction of cytochrome P450 1A1 (P450 1A1) in a variety of tissues is a well established consequence of exposure to 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD) and related compounds. Although localization of the induced protein within the lung has been described, the precise intracellular distribution of the enzyme is not clear. Analysis of tissue sections, microsomal proteins, and mRNA from lungs of treated and untreated rabbits established that P450 1A1 had been induced by treatment with TCDD. Rabbit lungs from animals treated with TCDD were examined with immunocytochemistry and in situ hybridization, to identify the cell types that contain P450 1A1 and those that contain mRNA encoding P450 1A1. Endothelial cells of the entire vascular bed of rabbit lung reacted markedly with anti-P450 1A1. Likewise, cells lining both arteries and veins, as well as capillary endothelial cells, reacted strongly with the cDNA probe for mRNA encoding P450 1A1. Clara cells at all levels of airway labeled prominently for both P- 450 1A1 and P450 1A1 mRNA. In addition, type 2 cells, alveolar macrophages, and, to a lesser degree, ciliated cells reacted with the cDNA probe. P450 reductase, which is required for P450 activity, has previously been identified in Clara cells, type 2 cells, and alveolar macrophages, but not in endothelium of rabbit lung. We have now obtained similar results for the localization of mRNA encoding P-450 reductase. This finding brings into question the function of P450 1A1 in endothelium.

Original languageEnglish (US)
Pages (from-to)1039-1046
Number of pages8
JournalMolecular Pharmacology
Volume41
Issue number6
StatePublished - Dec 1 1992
Externally publishedYes

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NADPH-Ferrihemoprotein Reductase
Cytochrome P-450 Enzyme System
In Situ Hybridization
Rabbits
Lung
Messenger RNA
Alveolar Macrophages
Endothelium
Oxidoreductases
Endothelial Cells
Complementary DNA
Polychlorinated Dibenzodioxins
Veins
Proteins
Arteries
Immunohistochemistry
Enzymes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Distribution of cytochrome P450 1A1 and NADPH-cytochrome P450 reductase in lungs of rabbits treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin : Ultrastructural immunolocalization and in situ hybridization. / Overby, L. H.; Nishio, S.; Weir, A.; Carver, G. T.; Plopper, Charles; Philpot, R. M.

In: Molecular Pharmacology, Vol. 41, No. 6, 01.12.1992, p. 1039-1046.

Research output: Contribution to journalArticle

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abstract = "Induction of cytochrome P450 1A1 (P450 1A1) in a variety of tissues is a well established consequence of exposure to 2,3,7,8-tetrachlorodibenzo-p- dioxin (TCDD) and related compounds. Although localization of the induced protein within the lung has been described, the precise intracellular distribution of the enzyme is not clear. Analysis of tissue sections, microsomal proteins, and mRNA from lungs of treated and untreated rabbits established that P450 1A1 had been induced by treatment with TCDD. Rabbit lungs from animals treated with TCDD were examined with immunocytochemistry and in situ hybridization, to identify the cell types that contain P450 1A1 and those that contain mRNA encoding P450 1A1. Endothelial cells of the entire vascular bed of rabbit lung reacted markedly with anti-P450 1A1. Likewise, cells lining both arteries and veins, as well as capillary endothelial cells, reacted strongly with the cDNA probe for mRNA encoding P450 1A1. Clara cells at all levels of airway labeled prominently for both P- 450 1A1 and P450 1A1 mRNA. In addition, type 2 cells, alveolar macrophages, and, to a lesser degree, ciliated cells reacted with the cDNA probe. P450 reductase, which is required for P450 activity, has previously been identified in Clara cells, type 2 cells, and alveolar macrophages, but not in endothelium of rabbit lung. We have now obtained similar results for the localization of mRNA encoding P-450 reductase. This finding brings into question the function of P450 1A1 in endothelium.",
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