Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice

T. C. Wu, Yu-Jui Yvonne Wan, I. Damjanov

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Fluorescein isothiocyanate labelled type I lectin from Bandeiraea simplicifolia (BSA-I) known for its specific binding to α-d-galactopyranosyl and 2-acetamido-2-deoxy-d-galactose groups, has been used to map the distribution of the lectin specific binding sites in the genital organs of female and male mice. In non-pregnant female mice, strong lectin reactivity was restricted to the epithelium of the distal oviduct, the cervix and vagina. In pregnant mice strong BSA-I reactivity was also noted in the epithelium of uterine glands from the time of implantation on day 5 onward. In the testis BSA-I bound selectively to sperm but did not react with other cells in the seminiferous tubules. In the proximal caput epididymis BSA-I reacted with the epithelial cells, the underlying basement membranes and the intraluminal sperm. The intraluminal contents of the seminal vesicles reacted strongly with the lectin. Our data thus show a widespread but selective distribution of BSA-I lectin binding sites in the male and female genital organs and altered lectin binding in the uterus during pregnancy.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalHistochemistry
Volume77
Issue number2
DOIs
StatePublished - Jun 1983
Externally publishedYes

Fingerprint

Griffonia simplicifolia
Male Genitalia
Female Genitalia
Lectins
lectins
genitalia
binding sites
Binding Sites
mice
Spermatozoa
Epithelium
epithelium
spermatozoa
Seminiferous Tubules
Oviducts
Seminal Vesicles
Epididymis
Vagina
Fluorescein
Galactose

ASJC Scopus subject areas

  • Anatomy

Cite this

Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice. / Wu, T. C.; Wan, Yu-Jui Yvonne; Damjanov, I.

In: Histochemistry, Vol. 77, No. 2, 06.1983, p. 233-241.

Research output: Contribution to journalArticle

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