Abstract
Fluorescein isothiocyanate labelled type I lectin from Bandeiraea simplicifolia (BSA-I) known for its specific binding to α-d-galactopyranosyl and 2-acetamido-2-deoxy-d-galactose groups, has been used to map the distribution of the lectin specific binding sites in the genital organs of female and male mice. In non-pregnant female mice, strong lectin reactivity was restricted to the epithelium of the distal oviduct, the cervix and vagina. In pregnant mice strong BSA-I reactivity was also noted in the epithelium of uterine glands from the time of implantation on day 5 onward. In the testis BSA-I bound selectively to sperm but did not react with other cells in the seminiferous tubules. In the proximal caput epididymis BSA-I reacted with the epithelial cells, the underlying basement membranes and the intraluminal sperm. The intraluminal contents of the seminal vesicles reacted strongly with the lectin. Our data thus show a widespread but selective distribution of BSA-I lectin binding sites in the male and female genital organs and altered lectin binding in the uterus during pregnancy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-241 |
| Number of pages | 9 |
| Journal | Histochemistry |
| Volume | 77 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Anatomy
Cite this
Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice. / Wu, T. C.; Wan, Yu-Jui Yvonne; Damjanov, I.
In: Histochemistry, Vol. 77, No. 2, 06.1983, p. 233-241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Distribution of Bandeiraea simplicifolia lectin binding sites in the genital organs of female and male mice
AU - Wu, T. C.
AU - Wan, Yu-Jui Yvonne
AU - Damjanov, I.
PY - 1983/6
Y1 - 1983/6
N2 - Fluorescein isothiocyanate labelled type I lectin from Bandeiraea simplicifolia (BSA-I) known for its specific binding to α-d-galactopyranosyl and 2-acetamido-2-deoxy-d-galactose groups, has been used to map the distribution of the lectin specific binding sites in the genital organs of female and male mice. In non-pregnant female mice, strong lectin reactivity was restricted to the epithelium of the distal oviduct, the cervix and vagina. In pregnant mice strong BSA-I reactivity was also noted in the epithelium of uterine glands from the time of implantation on day 5 onward. In the testis BSA-I bound selectively to sperm but did not react with other cells in the seminiferous tubules. In the proximal caput epididymis BSA-I reacted with the epithelial cells, the underlying basement membranes and the intraluminal sperm. The intraluminal contents of the seminal vesicles reacted strongly with the lectin. Our data thus show a widespread but selective distribution of BSA-I lectin binding sites in the male and female genital organs and altered lectin binding in the uterus during pregnancy.
AB - Fluorescein isothiocyanate labelled type I lectin from Bandeiraea simplicifolia (BSA-I) known for its specific binding to α-d-galactopyranosyl and 2-acetamido-2-deoxy-d-galactose groups, has been used to map the distribution of the lectin specific binding sites in the genital organs of female and male mice. In non-pregnant female mice, strong lectin reactivity was restricted to the epithelium of the distal oviduct, the cervix and vagina. In pregnant mice strong BSA-I reactivity was also noted in the epithelium of uterine glands from the time of implantation on day 5 onward. In the testis BSA-I bound selectively to sperm but did not react with other cells in the seminiferous tubules. In the proximal caput epididymis BSA-I reacted with the epithelial cells, the underlying basement membranes and the intraluminal sperm. The intraluminal contents of the seminal vesicles reacted strongly with the lectin. Our data thus show a widespread but selective distribution of BSA-I lectin binding sites in the male and female genital organs and altered lectin binding in the uterus during pregnancy.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020581590&partnerID=8YFLogxK
U2 - 10.1007/BF00506566
DO - 10.1007/BF00506566
M3 - Article
C2 - 6341328
AN - SCOPUS:0020581590
VL - 77
SP - 233
EP - 241
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 2
ER -