Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues

A. A. Lackner, M. H. Rodriguez, C. E. Bush, R. J. Munn, H. S. Kwang, Peter F Moore, K. G. Osborn, P. A. Marx, M. B. Gardner, Linda J Lowenstine

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Simian acquired immune deficiency syndrome (SAIDS) in rhesus macaques (Macaca mulatta) at the California Primate Research Center is caused by a type D retrovirus designated SAIDS retrovirus serotype 1 (SRV-1). This syndrome is characterized by profound immunosuppression and death associated with opportunistic infections. Neurologic signs and lesions have not been described as part of this syndrome. The distribution of SRV-1 in the salivary glands, lymph nodes, spleens, thymuses, and brains of eight virus-infected rhesus macaques was examined by immunohistochemistry. Electron microscopy, in situ RNA hybridization and Southern blot hybridization were also performed on selected tissues to detect viral particles, RNA, and DNA, respectively. In seven of eight SRV-1-infected animals, the transmembrane envelope glycoprotein (gp20) of SRV-1 was present in three or more tissues, but never in the brain. In the remaining animal, no viral antigen was detected in any tissue. In this same group of animals, viral nucleic acid was detected in the lymph nodes of six of six animals by Southern blot hybridization, in the salivary glands of two of five animals by both Southern blot and in situ hybridizations, and, surprisingly, in the brains of three of three animals by Southern blot and of three of five animals by in situ hybridization, including the one animal in which viral gp20 was undetectable. None of these animals had neurologic signs or lesions. The detection of viral nucleic acid in the absence of viral antigen in the brain suggests latent SRV-1 infection of the central nervous system.

Original languageEnglish (US)
Pages (from-to)2134-2142
Number of pages9
JournalJournal of Virology
Volume62
Issue number6
StatePublished - 1988

Fingerprint

Betaretrovirus
immunosuppressive agents
Simian Acquired Immunodeficiency Syndrome
Macaca
Lymphoid Tissue
Immunosuppressive Agents
Retroviridae
Southern Blotting
serotypes
animals
Southern blotting
Macaca mulatta
In Situ Hybridization
brain
acquired immunodeficiency syndrome
Viral Antigens
viral antigens
Brain
Neurologic Manifestations
salivary glands

ASJC Scopus subject areas

  • Immunology

Cite this

Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues. / Lackner, A. A.; Rodriguez, M. H.; Bush, C. E.; Munn, R. J.; Kwang, H. S.; Moore, Peter F; Osborn, K. G.; Marx, P. A.; Gardner, M. B.; Lowenstine, Linda J.

In: Journal of Virology, Vol. 62, No. 6, 1988, p. 2134-2142.

Research output: Contribution to journalArticle

Lackner, AA, Rodriguez, MH, Bush, CE, Munn, RJ, Kwang, HS, Moore, PF, Osborn, KG, Marx, PA, Gardner, MB & Lowenstine, LJ 1988, 'Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues', Journal of Virology, vol. 62, no. 6, pp. 2134-2142.
Lackner, A. A. ; Rodriguez, M. H. ; Bush, C. E. ; Munn, R. J. ; Kwang, H. S. ; Moore, Peter F ; Osborn, K. G. ; Marx, P. A. ; Gardner, M. B. ; Lowenstine, Linda J. / Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues. In: Journal of Virology. 1988 ; Vol. 62, No. 6. pp. 2134-2142.
@article{cb276ccad6a445a39d742a2bd1e58a1b,
title = "Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues",
abstract = "Simian acquired immune deficiency syndrome (SAIDS) in rhesus macaques (Macaca mulatta) at the California Primate Research Center is caused by a type D retrovirus designated SAIDS retrovirus serotype 1 (SRV-1). This syndrome is characterized by profound immunosuppression and death associated with opportunistic infections. Neurologic signs and lesions have not been described as part of this syndrome. The distribution of SRV-1 in the salivary glands, lymph nodes, spleens, thymuses, and brains of eight virus-infected rhesus macaques was examined by immunohistochemistry. Electron microscopy, in situ RNA hybridization and Southern blot hybridization were also performed on selected tissues to detect viral particles, RNA, and DNA, respectively. In seven of eight SRV-1-infected animals, the transmembrane envelope glycoprotein (gp20) of SRV-1 was present in three or more tissues, but never in the brain. In the remaining animal, no viral antigen was detected in any tissue. In this same group of animals, viral nucleic acid was detected in the lymph nodes of six of six animals by Southern blot hybridization, in the salivary glands of two of five animals by both Southern blot and in situ hybridizations, and, surprisingly, in the brains of three of three animals by Southern blot and of three of five animals by in situ hybridization, including the one animal in which viral gp20 was undetectable. None of these animals had neurologic signs or lesions. The detection of viral nucleic acid in the absence of viral antigen in the brain suggests latent SRV-1 infection of the central nervous system.",
author = "Lackner, {A. A.} and Rodriguez, {M. H.} and Bush, {C. E.} and Munn, {R. J.} and Kwang, {H. S.} and Moore, {Peter F} and Osborn, {K. G.} and Marx, {P. A.} and Gardner, {M. B.} and Lowenstine, {Linda J}",
year = "1988",
language = "English (US)",
volume = "62",
pages = "2134--2142",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Distribution of a macaque immunosuppressive type D retrovirus in neural, lymphoid, and salivary tissues

AU - Lackner, A. A.

AU - Rodriguez, M. H.

AU - Bush, C. E.

AU - Munn, R. J.

AU - Kwang, H. S.

AU - Moore, Peter F

AU - Osborn, K. G.

AU - Marx, P. A.

AU - Gardner, M. B.

AU - Lowenstine, Linda J

PY - 1988

Y1 - 1988

N2 - Simian acquired immune deficiency syndrome (SAIDS) in rhesus macaques (Macaca mulatta) at the California Primate Research Center is caused by a type D retrovirus designated SAIDS retrovirus serotype 1 (SRV-1). This syndrome is characterized by profound immunosuppression and death associated with opportunistic infections. Neurologic signs and lesions have not been described as part of this syndrome. The distribution of SRV-1 in the salivary glands, lymph nodes, spleens, thymuses, and brains of eight virus-infected rhesus macaques was examined by immunohistochemistry. Electron microscopy, in situ RNA hybridization and Southern blot hybridization were also performed on selected tissues to detect viral particles, RNA, and DNA, respectively. In seven of eight SRV-1-infected animals, the transmembrane envelope glycoprotein (gp20) of SRV-1 was present in three or more tissues, but never in the brain. In the remaining animal, no viral antigen was detected in any tissue. In this same group of animals, viral nucleic acid was detected in the lymph nodes of six of six animals by Southern blot hybridization, in the salivary glands of two of five animals by both Southern blot and in situ hybridizations, and, surprisingly, in the brains of three of three animals by Southern blot and of three of five animals by in situ hybridization, including the one animal in which viral gp20 was undetectable. None of these animals had neurologic signs or lesions. The detection of viral nucleic acid in the absence of viral antigen in the brain suggests latent SRV-1 infection of the central nervous system.

AB - Simian acquired immune deficiency syndrome (SAIDS) in rhesus macaques (Macaca mulatta) at the California Primate Research Center is caused by a type D retrovirus designated SAIDS retrovirus serotype 1 (SRV-1). This syndrome is characterized by profound immunosuppression and death associated with opportunistic infections. Neurologic signs and lesions have not been described as part of this syndrome. The distribution of SRV-1 in the salivary glands, lymph nodes, spleens, thymuses, and brains of eight virus-infected rhesus macaques was examined by immunohistochemistry. Electron microscopy, in situ RNA hybridization and Southern blot hybridization were also performed on selected tissues to detect viral particles, RNA, and DNA, respectively. In seven of eight SRV-1-infected animals, the transmembrane envelope glycoprotein (gp20) of SRV-1 was present in three or more tissues, but never in the brain. In the remaining animal, no viral antigen was detected in any tissue. In this same group of animals, viral nucleic acid was detected in the lymph nodes of six of six animals by Southern blot hybridization, in the salivary glands of two of five animals by both Southern blot and in situ hybridizations, and, surprisingly, in the brains of three of three animals by Southern blot and of three of five animals by in situ hybridization, including the one animal in which viral gp20 was undetectable. None of these animals had neurologic signs or lesions. The detection of viral nucleic acid in the absence of viral antigen in the brain suggests latent SRV-1 infection of the central nervous system.

UR - http://www.scopus.com/inward/record.url?scp=0023921518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023921518&partnerID=8YFLogxK

M3 - Article

VL - 62

SP - 2134

EP - 2142

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 6

ER -