Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest: Utility of Environmental DNA Sampling

Christine A. Richey, Kirsten V. Kenelty, Kristina Van Stone Hopkins, Brittany N. Stevens, Beatriz Martinez Lopez, Samantha M. Barnum, Sascha L. Hallett, Stephen D. Atkinson, Jerri L. Bartholomew, Esteban Soto Martinez

Research output: Contribution to journalArticle

Abstract

Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M. cerebralis. Analysis of environmental DNA (eDNA) has the potential to be a powerful tool for evaluating the presence of microorganisms, for which direct observation is impossible. We investigated the presence of M. cerebraliseDNA in river water and river sediment samples collected from areas affected by recent fire activity in Plumas National Forest, California. We compared eDNA loads in the environment to M. cerebralis infection in T. tubifex and sentinel-exposed Rainbow Trout Oncorhynchus mykiss and the presence of T. tubifex lineages in the same environment. For the latter, we developed a multiplex quantitative PCR assay for detection of T. tubifex lineages I, III, and V. Lineage IIIT. tubifex and M. cerebralis (eDNA as well as DNA extracted from fish and worm tissues) were detected only in samples obtained from areas affected by the Moonlight wildfire. The association between M. cerebralis infection in sentinel-exposed fish and eDNA detection in environmental samples only approached significance at a P-value of 0.056. However, given the difference in relative effort between the two sampling methods (host versus nonhost environment), our data suggest that eDNA sampling of water and substrate is a promising approach for surveillance of myxozoan fish parasites.

Original languageEnglish (US)
JournalJournal of Aquatic Animal Health
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Plumas National Forest
Myxobolus cerebralis
Tubifex tubifex
DNA
sampling
parasite
Oligochaeta
fish
wildfires
wildfire
parasites
Oncorhynchus mykiss
whirling disease
Tubifex
Tubificidae
sediments
etiological agents
environmental assessment
stream channel
salmonid

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest : Utility of Environmental DNA Sampling. / Richey, Christine A.; Kenelty, Kirsten V.; Van Stone Hopkins, Kristina; Stevens, Brittany N.; Martinez Lopez, Beatriz; Barnum, Samantha M.; Hallett, Sascha L.; Atkinson, Stephen D.; Bartholomew, Jerri L.; Soto Martinez, Esteban.

In: Journal of Aquatic Animal Health, 01.01.2018.

Research output: Contribution to journalArticle

Richey, Christine A. ; Kenelty, Kirsten V. ; Van Stone Hopkins, Kristina ; Stevens, Brittany N. ; Martinez Lopez, Beatriz ; Barnum, Samantha M. ; Hallett, Sascha L. ; Atkinson, Stephen D. ; Bartholomew, Jerri L. ; Soto Martinez, Esteban. / Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest : Utility of Environmental DNA Sampling. In: Journal of Aquatic Animal Health. 2018.
@article{5611aaf563b74046944909aaf99c23e3,
title = "Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest: Utility of Environmental DNA Sampling",
abstract = "Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M. cerebralis. Analysis of environmental DNA (eDNA) has the potential to be a powerful tool for evaluating the presence of microorganisms, for which direct observation is impossible. We investigated the presence of M. cerebraliseDNA in river water and river sediment samples collected from areas affected by recent fire activity in Plumas National Forest, California. We compared eDNA loads in the environment to M. cerebralis infection in T. tubifex and sentinel-exposed Rainbow Trout Oncorhynchus mykiss and the presence of T. tubifex lineages in the same environment. For the latter, we developed a multiplex quantitative PCR assay for detection of T. tubifex lineages I, III, and V. Lineage IIIT. tubifex and M. cerebralis (eDNA as well as DNA extracted from fish and worm tissues) were detected only in samples obtained from areas affected by the Moonlight wildfire. The association between M. cerebralis infection in sentinel-exposed fish and eDNA detection in environmental samples only approached significance at a P-value of 0.056. However, given the difference in relative effort between the two sampling methods (host versus nonhost environment), our data suggest that eDNA sampling of water and substrate is a promising approach for surveillance of myxozoan fish parasites.",
author = "Richey, {Christine A.} and Kenelty, {Kirsten V.} and {Van Stone Hopkins}, Kristina and Stevens, {Brittany N.} and {Martinez Lopez}, Beatriz and Barnum, {Samantha M.} and Hallett, {Sascha L.} and Atkinson, {Stephen D.} and Bartholomew, {Jerri L.} and {Soto Martinez}, Esteban",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/aah.10014",
language = "English (US)",
journal = "Journal of Aquatic Animal Health",
issn = "0899-7659",
publisher = "American Fisheries Society",

}

TY - JOUR

T1 - Distribution and Prevalence of Myxobolus cerebralis in Postfire Areas of Plumas National Forest

T2 - Utility of Environmental DNA Sampling

AU - Richey, Christine A.

AU - Kenelty, Kirsten V.

AU - Van Stone Hopkins, Kristina

AU - Stevens, Brittany N.

AU - Martinez Lopez, Beatriz

AU - Barnum, Samantha M.

AU - Hallett, Sascha L.

AU - Atkinson, Stephen D.

AU - Bartholomew, Jerri L.

AU - Soto Martinez, Esteban

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M. cerebralis. Analysis of environmental DNA (eDNA) has the potential to be a powerful tool for evaluating the presence of microorganisms, for which direct observation is impossible. We investigated the presence of M. cerebraliseDNA in river water and river sediment samples collected from areas affected by recent fire activity in Plumas National Forest, California. We compared eDNA loads in the environment to M. cerebralis infection in T. tubifex and sentinel-exposed Rainbow Trout Oncorhynchus mykiss and the presence of T. tubifex lineages in the same environment. For the latter, we developed a multiplex quantitative PCR assay for detection of T. tubifex lineages I, III, and V. Lineage IIIT. tubifex and M. cerebralis (eDNA as well as DNA extracted from fish and worm tissues) were detected only in samples obtained from areas affected by the Moonlight wildfire. The association between M. cerebralis infection in sentinel-exposed fish and eDNA detection in environmental samples only approached significance at a P-value of 0.056. However, given the difference in relative effort between the two sampling methods (host versus nonhost environment), our data suggest that eDNA sampling of water and substrate is a promising approach for surveillance of myxozoan fish parasites.

AB - Myxobolus cerebralis is a myxozoan parasite and the etiological agent of whirling disease in salmonids. The parasite's life cycle involves waterborne spores and requires both a salmonid fish and the benthic freshwater oligochaete worm Tubifex tubifex (Oligochaeta: Tubificidae). Wildfires can lead to the erosion of fine sediments into stream channels and have been implicated as promoting environmental conditions that are suitable for the survival and success of T. tubifex, whose presence in turn can affect the prevalence of M. cerebralis. Analysis of environmental DNA (eDNA) has the potential to be a powerful tool for evaluating the presence of microorganisms, for which direct observation is impossible. We investigated the presence of M. cerebraliseDNA in river water and river sediment samples collected from areas affected by recent fire activity in Plumas National Forest, California. We compared eDNA loads in the environment to M. cerebralis infection in T. tubifex and sentinel-exposed Rainbow Trout Oncorhynchus mykiss and the presence of T. tubifex lineages in the same environment. For the latter, we developed a multiplex quantitative PCR assay for detection of T. tubifex lineages I, III, and V. Lineage IIIT. tubifex and M. cerebralis (eDNA as well as DNA extracted from fish and worm tissues) were detected only in samples obtained from areas affected by the Moonlight wildfire. The association between M. cerebralis infection in sentinel-exposed fish and eDNA detection in environmental samples only approached significance at a P-value of 0.056. However, given the difference in relative effort between the two sampling methods (host versus nonhost environment), our data suggest that eDNA sampling of water and substrate is a promising approach for surveillance of myxozoan fish parasites.

UR - http://www.scopus.com/inward/record.url?scp=85046076845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046076845&partnerID=8YFLogxK

U2 - 10.1002/aah.10014

DO - 10.1002/aah.10014

M3 - Article

C2 - 29710407

AN - SCOPUS:85046076845

JO - Journal of Aquatic Animal Health

JF - Journal of Aquatic Animal Health

SN - 0899-7659

ER -