Distinct tachykinin NK 1 receptor function in primate nucleus tractus solitarius neurons is dysregulated after second-hand tobacco smoke exposure

Shin Ichi Sekizawa, Jesse P. Joad, Kent E Pinkerton, Ann C. Bonham

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE Second-hand tobacco smoke (SHS) exposure in children increases the risk of asthma and sudden infant death syndrome. Epidemiological and experimental data have suggested SHS can alter neuroplasticity in the CNS, associated with substance P. We hypothesized that exposure to SHS in young primates changed the effect of substance P on the plasticity of neurons in the nucleus tractus solitarius (NTS), where airway sensory information is first processed in the CNS. EXPERIMENTAL APPROACH Thirteen-month-old rhesus monkeys were exposed to filtered air (FA, n= 5) or SHS (n= 5) for >6 months from 50 days of their fetal age. Whole-cell patch-clamp recordings were performed on NTS neurons in brainstem slices from these animals to record the intrinsic cell excitability in the absence or presence of the NK 1 receptor antagonist, SR140333 (3 μM). KEY RESULTS Neurons were electrophysiologically classified based on their spiking onset from a hyperpolarized membrane potential into two phenotypes: rapid-onset spiking (RS) and delayed-onset spiking (DS) types. In RS neurons, SR140333 reduced the spiking response, similarly in both FA- and SHS-exposed animals. In DS neurons, SR140333 almost abolished the spiking response in FA-exposed animals, but had no effect in SHS-exposed animals. CONCLUSIONS AND IMPLICATIONS The contribution of NK 1 receptors to cell excitability depended on firing phenotype of primate NTS neurons and was disrupted by SHS exposure, specifically in DS neurons. Our findings reveal a novel NK 1 receptor function in the primate brainstem and support the hypothesis that chronic exposure to SHS in children causes tachykinin-related neuroplastic changes in the CNS.

Original languageEnglish (US)
Pages (from-to)782-791
Number of pages10
JournalBritish Journal of Pharmacology
Volume163
Issue number4
DOIs
StatePublished - Jun 2011

Fingerprint

Neurokinin-1 Receptors
Tachykinins
Tobacco Smoke Pollution
Solitary Nucleus
Primates
Tobacco
Neurons
Substance P
Brain Stem
Phenotype
Neuronal Plasticity
Sudden Infant Death
Macaca mulatta
Membrane Potentials
Gestational Age
Asthma
Air

Keywords

  • air pollutants
  • intrinsic cell excitability
  • neurokinin 1 receptor
  • nucleus tractus solitarius
  • primates
  • substance P

ASJC Scopus subject areas

  • Pharmacology

Cite this

Distinct tachykinin NK 1 receptor function in primate nucleus tractus solitarius neurons is dysregulated after second-hand tobacco smoke exposure. / Sekizawa, Shin Ichi; Joad, Jesse P.; Pinkerton, Kent E; Bonham, Ann C.

In: British Journal of Pharmacology, Vol. 163, No. 4, 06.2011, p. 782-791.

Research output: Contribution to journalArticle

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abstract = "BACKGROUND AND PURPOSE Second-hand tobacco smoke (SHS) exposure in children increases the risk of asthma and sudden infant death syndrome. Epidemiological and experimental data have suggested SHS can alter neuroplasticity in the CNS, associated with substance P. We hypothesized that exposure to SHS in young primates changed the effect of substance P on the plasticity of neurons in the nucleus tractus solitarius (NTS), where airway sensory information is first processed in the CNS. EXPERIMENTAL APPROACH Thirteen-month-old rhesus monkeys were exposed to filtered air (FA, n= 5) or SHS (n= 5) for >6 months from 50 days of their fetal age. Whole-cell patch-clamp recordings were performed on NTS neurons in brainstem slices from these animals to record the intrinsic cell excitability in the absence or presence of the NK 1 receptor antagonist, SR140333 (3 μM). KEY RESULTS Neurons were electrophysiologically classified based on their spiking onset from a hyperpolarized membrane potential into two phenotypes: rapid-onset spiking (RS) and delayed-onset spiking (DS) types. In RS neurons, SR140333 reduced the spiking response, similarly in both FA- and SHS-exposed animals. In DS neurons, SR140333 almost abolished the spiking response in FA-exposed animals, but had no effect in SHS-exposed animals. CONCLUSIONS AND IMPLICATIONS The contribution of NK 1 receptors to cell excitability depended on firing phenotype of primate NTS neurons and was disrupted by SHS exposure, specifically in DS neurons. Our findings reveal a novel NK 1 receptor function in the primate brainstem and support the hypothesis that chronic exposure to SHS in children causes tachykinin-related neuroplastic changes in the CNS.",
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