Distinct spatial localization of specific mRNAs in cultured sympathetic neurons

David A. Bruckenstein, Pamela J Lein, Dennis Higgins, Robert T. Fremeau

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

We examined the subcellular distribution of specific mRNAs in cultured sympathetic neurons. Under appropriate conditions, sympathetic neurons extend both axons and dendrites that are distinguishable by light microscopic and immunocytochemical criteria. In situ hybridization revealed a differential localization of mRNA within dendrites. mRNA encoding MAP2 was abundant in cell bodies and distributed nonhomogeneously throughout the dendritic compartment, but was not detected in axons. In contrast, mRNAs encoding GAP-43 and a-tubulin were restricted to the cell body and largely excluded from dendrites as well as axons. Detergent extraction revealed that most dendrite-associated mRNA encoding MAP2 was associated with the Triton X-100 insoluble fraction of the cell. The subset of mRNAs present in the dendritic compartment may encode proteins involved in the morphogenesis and remodeling of dendrites.

Original languageEnglish (US)
Pages (from-to)809-819
Number of pages11
JournalNeuron
Volume5
Issue number6
DOIs
StatePublished - 1990
Externally publishedYes

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Dendrites
Neurons
Messenger RNA
Axons
GAP-43 Protein
Octoxynol
Tubulin
Morphogenesis
Detergents
In Situ Hybridization
Light
Proteins
Cell Body

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distinct spatial localization of specific mRNAs in cultured sympathetic neurons. / Bruckenstein, David A.; Lein, Pamela J; Higgins, Dennis; Fremeau, Robert T.

In: Neuron, Vol. 5, No. 6, 1990, p. 809-819.

Research output: Contribution to journalArticle

Bruckenstein, David A. ; Lein, Pamela J ; Higgins, Dennis ; Fremeau, Robert T. / Distinct spatial localization of specific mRNAs in cultured sympathetic neurons. In: Neuron. 1990 ; Vol. 5, No. 6. pp. 809-819.
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