Distinct routes of lineage development reshape the human blood hierarchy across ontogeny

Faiyaz Notta, Sasan Zandi, Naoya Takayama, Stephanie Dobson, Olga I. Gan, Gavin Wilson, Kerstin B. Kaufmann, Jessica McLeod, Elisa Laurenti, Cyrille F. Dunant, John Douglas Mcpherson, Lincoln D. Stein, Yigal Dror, John E. Dick

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

In a classical view of hematopoiesis, the various blood cell lineages arise via a hierarchical scheme starting with multipotent stem cells that become increasingly restricted in their differentiation potential through oligopotent and then unipotent progenitors. We developed a cell-sorting scheme to resolve myeloid (My), erythroid (Er), and megakaryocytic (Mk) fates from single CD34+ cells and then mapped the progenitor hierarchy across human development. Fetal liver contained large numbers of distinct oligopotent progenitors with intermingled My, Er, and Mk fates. However, few oligopotent progenitor intermediates were present in the adult bone marrow. Instead, only two progenitor classes predominate, multipotent and unipotent, with Er-Mk lineages emerging from multipotent cells. The developmental shift to an adult "two-tier" hierarchy challenges current dogma and provides a revised framework to understand normal and disease states of human hematopoiesis.

Original languageEnglish (US)
Article numberaab2116
JournalScience
Volume351
Issue number6269
DOIs
StatePublished - Jan 8 2016
Externally publishedYes

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Human Development
Hematopoiesis
Multipotent Stem Cells
Cell Lineage
Blood Cells
Bone Marrow
Liver

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Notta, F., Zandi, S., Takayama, N., Dobson, S., Gan, O. I., Wilson, G., ... Dick, J. E. (2016). Distinct routes of lineage development reshape the human blood hierarchy across ontogeny. Science, 351(6269), [aab2116]. https://doi.org/10.1126/science.aab2116

Distinct routes of lineage development reshape the human blood hierarchy across ontogeny. / Notta, Faiyaz; Zandi, Sasan; Takayama, Naoya; Dobson, Stephanie; Gan, Olga I.; Wilson, Gavin; Kaufmann, Kerstin B.; McLeod, Jessica; Laurenti, Elisa; Dunant, Cyrille F.; Mcpherson, John Douglas; Stein, Lincoln D.; Dror, Yigal; Dick, John E.

In: Science, Vol. 351, No. 6269, aab2116, 08.01.2016.

Research output: Contribution to journalArticle

Notta, F, Zandi, S, Takayama, N, Dobson, S, Gan, OI, Wilson, G, Kaufmann, KB, McLeod, J, Laurenti, E, Dunant, CF, Mcpherson, JD, Stein, LD, Dror, Y & Dick, JE 2016, 'Distinct routes of lineage development reshape the human blood hierarchy across ontogeny', Science, vol. 351, no. 6269, aab2116. https://doi.org/10.1126/science.aab2116
Notta F, Zandi S, Takayama N, Dobson S, Gan OI, Wilson G et al. Distinct routes of lineage development reshape the human blood hierarchy across ontogeny. Science. 2016 Jan 8;351(6269). aab2116. https://doi.org/10.1126/science.aab2116
Notta, Faiyaz ; Zandi, Sasan ; Takayama, Naoya ; Dobson, Stephanie ; Gan, Olga I. ; Wilson, Gavin ; Kaufmann, Kerstin B. ; McLeod, Jessica ; Laurenti, Elisa ; Dunant, Cyrille F. ; Mcpherson, John Douglas ; Stein, Lincoln D. ; Dror, Yigal ; Dick, John E. / Distinct routes of lineage development reshape the human blood hierarchy across ontogeny. In: Science. 2016 ; Vol. 351, No. 6269.
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