Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly

Elena Nazarova, Eileen O'Toole, Susi Kaitna, Paul Francois, Mark Winey, Jackie Vogel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

During spindle assembly, microtubules may attach to kinetochores or pair to form antiparallel pairs or interpolar microtubules, which span the two spindle poles and contribute to mitotic pole separation and chromosome segregation. Events in the specification of the interpolar microtubules are poorly understood. Using three-dimensional electron tomography and analysis of spindle dynamical behavior in living cells, we investigated the process of spindle assembly. Unexpectedly, we found that the phosphorylation state of an evolutionarily conserved Cdk1 site (S360) in γ-tubulin is correlated with the number and organization of interpolar microtubules. Mimicking S360 phosphorylation (S360D) results in bipolar spindles with a normal number of microtubules but lacking interpolar microtubules. Inhibiting S360 phosphorylation (S360A) results in spindles with interpolar microtubules and high-angle, antiparallel microtubule pairs. The latter are also detected in wild-type spindles <1 μm in length, suggesting that high-angle microtubule pairing represents an intermediate step in interpolar microtubule formation. Correlation of spindle architecture with dynamical behavior suggests that microtubule pairing is sufficient to separate the spindle poles, whereas interpolar microtubules maintain the velocity of pole displacement during early spindle assembly. Our findings suggest that the number of interpolar microtubules formed during spindle assembly is controlled in part through activities at the spindle poles.

Original languageEnglish (US)
Pages (from-to)3238-3250
Number of pages13
JournalMolecular Biology of the Cell
Volume24
Issue number20
DOIs
StatePublished - Oct 15 2013
Externally publishedYes

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Microtubules
Spindle Poles
Phosphorylation
Electron Microscope Tomography
Kinetochores
Chromosome Segregation
Tubulin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly. / Nazarova, Elena; O'Toole, Eileen; Kaitna, Susi; Francois, Paul; Winey, Mark; Vogel, Jackie.

In: Molecular Biology of the Cell, Vol. 24, No. 20, 15.10.2013, p. 3238-3250.

Research output: Contribution to journalArticle

Nazarova, Elena ; O'Toole, Eileen ; Kaitna, Susi ; Francois, Paul ; Winey, Mark ; Vogel, Jackie. / Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly. In: Molecular Biology of the Cell. 2013 ; Vol. 24, No. 20. pp. 3238-3250.
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