Distinct patterns of gene expression in the superficial, middle and deep zones of bovine articular cartilage

Derek F. Amanatullah, Shintaro Yamane, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Hyaline articular cartilage will not heal spontaneously, and lesions in hyaline articular cartilage often result in degenerative joint disease. Considerable progress has been made with respect to the responsive stem cells, inductive signals and extracellular scaffolding required for the optimal regeneration of cartilage. However, many challenges remain, such as topographic differences in the functional zones of articular cartilage. We hypothesized that a distinct set of differentially expressed genes define the surface, middle and deep zones of hyaline articular cartilage. Microarray analysis of bovine articular cartilage from the superficial and middle zones revealed 52 genes differentially expressed ≥ 10-fold and 114 additional genes differentially expressed ≥ five-fold. However, no genes were identified with a ≥ five-fold difference in expression when comparing articular cartilage from the middle and deep zones. There are distinct, differential gene expression patterns in the superficial and middle zones of hyaline articular cartilage that highlight the functional differences between these zones. This investigation has implications for the tissue engineering and regeneration of hyaline articular cartilage.

Original languageEnglish (US)
Pages (from-to)505-514
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number7
StatePublished - 2014


  • Cartilage
  • Differential
  • Expression
  • Gene
  • Microarray
  • Zone

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Medicine(all)


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