Distinct patterns of gene expression in the superficial, middle and deep zones of bovine articular cartilage

Derek F. Amanatullah, Shintaro Yamane, A Hari Reddi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Hyaline articular cartilage will not heal spontaneously, and lesions in hyaline articular cartilage often result in degenerative joint disease. Considerable progress has been made with respect to the responsive stem cells, inductive signals and extracellular scaffolding required for the optimal regeneration of cartilage. However, many challenges remain, such as topographic differences in the functional zones of articular cartilage. We hypothesized that a distinct set of differentially expressed genes define the surface, middle and deep zones of hyaline articular cartilage. Microarray analysis of bovine articular cartilage from the superficial and middle zones revealed 52 genes differentially expressed ≥ 10-fold and 114 additional genes differentially expressed ≥ five-fold. However, no genes were identified with a ≥ five-fold difference in expression when comparing articular cartilage from the middle and deep zones. There are distinct, differential gene expression patterns in the superficial and middle zones of hyaline articular cartilage that highlight the functional differences between these zones. This investigation has implications for the tissue engineering and regeneration of hyaline articular cartilage.

Original languageEnglish (US)
Pages (from-to)505-514
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume8
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Cartilage
Articular Cartilage
Gene expression
Hyaline Cartilage
Gene Expression
Genes
Regeneration
Tissue regeneration
Tissue Engineering
Microarray Analysis
Osteoarthritis
Microarrays
Stem cells
Tissue engineering
Stem Cells

Keywords

  • Cartilage
  • Differential
  • Expression
  • Gene
  • Microarray
  • Zone

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Medicine(all)

Cite this

Distinct patterns of gene expression in the superficial, middle and deep zones of bovine articular cartilage. / Amanatullah, Derek F.; Yamane, Shintaro; Reddi, A Hari.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 8, No. 7, 2014, p. 505-514.

Research output: Contribution to journalArticle

@article{1dc745df27434a3b807ea0d190b0cb8b,
title = "Distinct patterns of gene expression in the superficial, middle and deep zones of bovine articular cartilage",
abstract = "Hyaline articular cartilage will not heal spontaneously, and lesions in hyaline articular cartilage often result in degenerative joint disease. Considerable progress has been made with respect to the responsive stem cells, inductive signals and extracellular scaffolding required for the optimal regeneration of cartilage. However, many challenges remain, such as topographic differences in the functional zones of articular cartilage. We hypothesized that a distinct set of differentially expressed genes define the surface, middle and deep zones of hyaline articular cartilage. Microarray analysis of bovine articular cartilage from the superficial and middle zones revealed 52 genes differentially expressed ≥ 10-fold and 114 additional genes differentially expressed ≥ five-fold. However, no genes were identified with a ≥ five-fold difference in expression when comparing articular cartilage from the middle and deep zones. There are distinct, differential gene expression patterns in the superficial and middle zones of hyaline articular cartilage that highlight the functional differences between these zones. This investigation has implications for the tissue engineering and regeneration of hyaline articular cartilage.",
keywords = "Cartilage, Differential, Expression, Gene, Microarray, Zone",
author = "Amanatullah, {Derek F.} and Shintaro Yamane and Reddi, {A Hari}",
year = "2014",
doi = "10.1002/term.1543",
language = "English (US)",
volume = "8",
pages = "505--514",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

TY - JOUR

T1 - Distinct patterns of gene expression in the superficial, middle and deep zones of bovine articular cartilage

AU - Amanatullah, Derek F.

AU - Yamane, Shintaro

AU - Reddi, A Hari

PY - 2014

Y1 - 2014

N2 - Hyaline articular cartilage will not heal spontaneously, and lesions in hyaline articular cartilage often result in degenerative joint disease. Considerable progress has been made with respect to the responsive stem cells, inductive signals and extracellular scaffolding required for the optimal regeneration of cartilage. However, many challenges remain, such as topographic differences in the functional zones of articular cartilage. We hypothesized that a distinct set of differentially expressed genes define the surface, middle and deep zones of hyaline articular cartilage. Microarray analysis of bovine articular cartilage from the superficial and middle zones revealed 52 genes differentially expressed ≥ 10-fold and 114 additional genes differentially expressed ≥ five-fold. However, no genes were identified with a ≥ five-fold difference in expression when comparing articular cartilage from the middle and deep zones. There are distinct, differential gene expression patterns in the superficial and middle zones of hyaline articular cartilage that highlight the functional differences between these zones. This investigation has implications for the tissue engineering and regeneration of hyaline articular cartilage.

AB - Hyaline articular cartilage will not heal spontaneously, and lesions in hyaline articular cartilage often result in degenerative joint disease. Considerable progress has been made with respect to the responsive stem cells, inductive signals and extracellular scaffolding required for the optimal regeneration of cartilage. However, many challenges remain, such as topographic differences in the functional zones of articular cartilage. We hypothesized that a distinct set of differentially expressed genes define the surface, middle and deep zones of hyaline articular cartilage. Microarray analysis of bovine articular cartilage from the superficial and middle zones revealed 52 genes differentially expressed ≥ 10-fold and 114 additional genes differentially expressed ≥ five-fold. However, no genes were identified with a ≥ five-fold difference in expression when comparing articular cartilage from the middle and deep zones. There are distinct, differential gene expression patterns in the superficial and middle zones of hyaline articular cartilage that highlight the functional differences between these zones. This investigation has implications for the tissue engineering and regeneration of hyaline articular cartilage.

KW - Cartilage

KW - Differential

KW - Expression

KW - Gene

KW - Microarray

KW - Zone

UR - http://www.scopus.com/inward/record.url?scp=84903814670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903814670&partnerID=8YFLogxK

U2 - 10.1002/term.1543

DO - 10.1002/term.1543

M3 - Article

C2 - 22777751

AN - SCOPUS:84903814670

VL - 8

SP - 505

EP - 514

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 7

ER -