Distinct cross-reactive B-cell responses to live attenuated and inactivated influenza vaccines

Sanae Sasaki, Tyson H. Holmes, Randy A. Albrecht, Adolfo García-Sastre, Cornelia L. Dekker, Xiaosong He, Harry B. Greenberg

Research output: Contribution to journalArticle

23 Scopus citations


The immunological bases for the efficacies of the 2 currently licensed influenza vaccines, live attenuated influenza vaccine (LAIV) and inactivated influenza vaccine (IIV), are not fully understood. The goal of this study was to identify specific B-cell responses correlated with the known efficacies of these 2 vaccines. Methods. We compared the B-cell and antibody responses after immunization with 2010/2011 IIV or LAIV in young adults, focusing on peripheral plasmablasts 6-8 days after vaccination. Results. The quantities of vaccine-specific plasmablasts and plasmablast-derived polyclonal antibodies (PPAbs) in IIV recipients were significantly higher than those in LAIV recipients. No significant difference was detected in the avidity of vaccine-specific PPAbs between the 2 vaccine groups. Proportionally, LAIV induced a greater vaccinespecific immunoglobulin A plasmablast response, as well as a greater plasmablast response to the conserved influenza nuclear protein, than IIV. The cross-reactive plasmablast response to heterovariant strains, as indicated by the relative levels of cross-reactive plasmablasts and the cross-reactive PPAb binding reactivity, was also greater in the LAIV group. Conclusions. Distinct quantitative and qualitative patterns of plasmablast responses were induced by LAIV and IIV in young adults; a proportionally greater cross-reactive response was induced by LAIV.

Original languageEnglish (US)
Pages (from-to)865-874
Number of pages10
JournalJournal of Infectious Diseases
Issue number6
StatePublished - 2014
Externally publishedYes


  • Antibody
  • B-cell response
  • Influenza vaccine

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

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    Sasaki, S., Holmes, T. H., Albrecht, R. A., García-Sastre, A., Dekker, C. L., He, X., & Greenberg, H. B. (2014). Distinct cross-reactive B-cell responses to live attenuated and inactivated influenza vaccines. Journal of Infectious Diseases, 210(6), 865-874. https://doi.org/10.1093/infdis/jiu190