Distinct chemokine triggers and in vivo migratory paths of fluorescein dye-labeled T lymphocytes in acutely simian immunodeficiency virus SIV mac251-infected and uninfected macaques

Candice C. Clay, Denise S. Rodrigues, Danielle J Harvey, Christian M. Leutenegger, Ursula Esser

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To define the possible impact of T-lymphocyte trafficking parameters on simian immunodeficiency virus (SIV) pathogenesis, we examined migratory profiles of carboxyfluorescein diacetate seccinimidyl ester (CFSE)-labeled T lymphocytes in acutely SIVmac251-infected and uninfected macaques within 48 h after autologous transfer. Despite significant upregulation of homeostatic chemokine CCL19/macrophage inflammatory protein 3β and proinflammatory chemokine CXCL9/monokine induced by gamma interferon in secondary lymphoid tissue in SIV infection, no differences in CFSE+ T-lymphocyte frequencies or cell compartmentalization in lymph nodes were identified between animal groups. By contrast, a higher frequency of CFSE+ T lymphocytes in the small intestine was detected in acute SIV infection. This result correlated with increased numbers of gut CD4 T lymphocytes expressing chemokine receptors CCR9, CCR7, and CXCR3 and high levels of their respective chemokine ligands in the small intestine. The changes in trafficking parameters in SIV-infected macaques occurred concomitantly with acute gut CD4 T-lymphocyte depletion. Here, we present the first in vivo T-lymphocyte trafficking study in SIV infection and a novel approach to delineate T-lymphocyte recruitment into tissues in the nonhuman primate animal model for AIDS. Such studies are likely to provide unique insights into T-lymphocyte sequestration in distinct tissue compartments and possible mechanisms of CD4 T-lymphocyte depletion and immune dysfunction in simian AIDS.

Original languageEnglish (US)
Pages (from-to)13759-13768
Number of pages10
JournalJournal of Virology
Volume79
Issue number21
DOIs
StatePublished - Nov 2005

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca
chemokines
fluorescein
Fluorescein
Chemokines
dyes
Coloring Agents
T-lymphocytes
T-Lymphocytes
Virus Diseases
Lymphocyte Depletion
esters
Esters
small intestine
Small Intestine
digestive system
Simian Acquired Immunodeficiency Syndrome
animal models

ASJC Scopus subject areas

  • Immunology

Cite this

Distinct chemokine triggers and in vivo migratory paths of fluorescein dye-labeled T lymphocytes in acutely simian immunodeficiency virus SIV mac251-infected and uninfected macaques. / Clay, Candice C.; Rodrigues, Denise S.; Harvey, Danielle J; Leutenegger, Christian M.; Esser, Ursula.

In: Journal of Virology, Vol. 79, No. 21, 11.2005, p. 13759-13768.

Research output: Contribution to journalArticle

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