Distinct cellular receptor interactions in poliovirus and rhinoviruses

Li Xing, Karin Tjarnlund, Birgitta Lindqvist, Gerardo G. Kaplan, Dino Feigelstock, R. Holland Cheng, José M. Casasnovas

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Receptor binding to human poliovirus type 1 (PV1/M) and the major group of human rhinoviruses (HRV) was studied comparatively to uncover the evolution of receptor recognition in picornaviruses. Surface plasmon resonance showed receptor binding to PV1/M with faster association and dissociation rates than to HRV3 and HRV16, two serotypes that have similar binding kinetics. The faster rate for receptor association to PV1/M suggested a relatively more accessible binding site. Thermodynamics for receptor binding to the viruses and assays for receptor-mediated virus uncoating showed a more disruptive receptor interaction with PV1/M than with HRV3 or HRV16. Cryoelectron microscopy and image reconstruction of receptor-PV1/M complexes revealed receptor binding to the 'wall' of surface protrusions surrounding the 'canyon', a depressive surface in the capsid where the rhinovirus receptor binds. These data reveal more exposed receptor-binding sites in poliovirus than rhinoviruses, which are less protected from immune surveillance but more suited for receptor-mediated virus uncoating and entry at the cell surface.

Original languageEnglish (US)
Pages (from-to)1207-1216
Number of pages10
JournalEMBO Journal
Volume19
Issue number6
StatePublished - Mar 15 2000
Externally publishedYes

Fingerprint

Rhinovirus
Poliovirus
Virus Uncoating
Viruses
Binding Sites
Association reactions
Picornaviridae
Virus Receptors
Cryoelectron Microscopy
Virus Internalization
Computer-Assisted Image Processing
Surface Plasmon Resonance
Capsid
Surface plasmon resonance
Image reconstruction
Thermodynamics
Assays
Microscopic examination
Kinetics

Keywords

  • Cryo-EM and image reconstruction
  • Picornavirus entry
  • Poliovirus
  • Rhinovirus
  • Virus-recept interaction

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Xing, L., Tjarnlund, K., Lindqvist, B., Kaplan, G. G., Feigelstock, D., Cheng, R. H., & Casasnovas, J. M. (2000). Distinct cellular receptor interactions in poliovirus and rhinoviruses. EMBO Journal, 19(6), 1207-1216.

Distinct cellular receptor interactions in poliovirus and rhinoviruses. / Xing, Li; Tjarnlund, Karin; Lindqvist, Birgitta; Kaplan, Gerardo G.; Feigelstock, Dino; Cheng, R. Holland; Casasnovas, José M.

In: EMBO Journal, Vol. 19, No. 6, 15.03.2000, p. 1207-1216.

Research output: Contribution to journalArticle

Xing, L, Tjarnlund, K, Lindqvist, B, Kaplan, GG, Feigelstock, D, Cheng, RH & Casasnovas, JM 2000, 'Distinct cellular receptor interactions in poliovirus and rhinoviruses', EMBO Journal, vol. 19, no. 6, pp. 1207-1216.
Xing L, Tjarnlund K, Lindqvist B, Kaplan GG, Feigelstock D, Cheng RH et al. Distinct cellular receptor interactions in poliovirus and rhinoviruses. EMBO Journal. 2000 Mar 15;19(6):1207-1216.
Xing, Li ; Tjarnlund, Karin ; Lindqvist, Birgitta ; Kaplan, Gerardo G. ; Feigelstock, Dino ; Cheng, R. Holland ; Casasnovas, José M. / Distinct cellular receptor interactions in poliovirus and rhinoviruses. In: EMBO Journal. 2000 ; Vol. 19, No. 6. pp. 1207-1216.
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