Abstract
Implantation of demineralized tooth matrix in subcutaneous sites results in new bone formation locally. The osteoinductive activity of the tooth matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the residue was devoid of biologic activity. The bone inductive protein, osteogenin, was partially purified by heparin affinity chromatography. The heparin binding fraction initiated the bone differentiation cascade when implanted with guanidine extracted, inactive bone or tooth matrices. These results imply a cooperative interaction between the soluble osteogenin and collagenous substratum in bone induction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1253-1257 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 157 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 30 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Dissociative extraction and partial purification of osteogenin, a bone inductive protein, from rat tooth matrix by heparin affinity chromatography. / Katz, R. W.; Reddi, A Hari.
In: Biochemical and Biophysical Research Communications, Vol. 157, No. 3, 30.12.1988, p. 1253-1257.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dissociative extraction and partial purification of osteogenin, a bone inductive protein, from rat tooth matrix by heparin affinity chromatography
AU - Katz, R. W.
AU - Reddi, A Hari
PY - 1988/12/30
Y1 - 1988/12/30
N2 - Implantation of demineralized tooth matrix in subcutaneous sites results in new bone formation locally. The osteoinductive activity of the tooth matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the residue was devoid of biologic activity. The bone inductive protein, osteogenin, was partially purified by heparin affinity chromatography. The heparin binding fraction initiated the bone differentiation cascade when implanted with guanidine extracted, inactive bone or tooth matrices. These results imply a cooperative interaction between the soluble osteogenin and collagenous substratum in bone induction.
AB - Implantation of demineralized tooth matrix in subcutaneous sites results in new bone formation locally. The osteoinductive activity of the tooth matrix was dissociatively extracted in 4.0 M guanidine hydrochloride and the residue was devoid of biologic activity. The bone inductive protein, osteogenin, was partially purified by heparin affinity chromatography. The heparin binding fraction initiated the bone differentiation cascade when implanted with guanidine extracted, inactive bone or tooth matrices. These results imply a cooperative interaction between the soluble osteogenin and collagenous substratum in bone induction.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024205603&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81009-X
DO - 10.1016/S0006-291X(88)81009-X
M3 - Article
C2 - 3207424
AN - SCOPUS:0024205603
VL - 157
SP - 1253
EP - 1257
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -