Dissociation of functional roles of dynamin in receptor-mediated endocytosis and mitogenic signal transduction

Jennifer Whistler, Mark Von Zastrow

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Dynamin plays a critical role in the membrane fission mechanism that mediates regulated endocytosis of many G protein-coupled receptors. In addition, dynamin is required for ligand-induced activation of mitogen- activated protein kinase by certain receptors, raising a general question about the role of dynamin in mitogenic signal transduction. Here we report that endocytosis of μ and δ opioid receptors is not required for efficient ligand-induced activation of mitogen-activated protein kinase. Nevertheless, mitogenic signaling mediated by these receptors is specifically dynamin- dependent. Thus a functional role of dynamin in mitogenic signaling can be dissociated from its role in receptor-mediated endocytosis, suggesting a previously unidentified and distinct role of dynamin in signal transduction by certain G protein-coupled receptors.

Original languageEnglish (US)
Pages (from-to)24575-24578
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number35
DOIs
StatePublished - Aug 27 1999
Externally publishedYes

Fingerprint

Dynamins
Signal transduction
Endocytosis
Signal Transduction
G-Protein-Coupled Receptors
Mitogen-Activated Protein Kinases
Chemical activation
Ligands
Opioid Receptors
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dissociation of functional roles of dynamin in receptor-mediated endocytosis and mitogenic signal transduction. / Whistler, Jennifer; Von Zastrow, Mark.

In: Journal of Biological Chemistry, Vol. 274, No. 35, 27.08.1999, p. 24575-24578.

Research output: Contribution to journalArticle

@article{6503f6a585834048b257fd219075be14,
title = "Dissociation of functional roles of dynamin in receptor-mediated endocytosis and mitogenic signal transduction",
abstract = "Dynamin plays a critical role in the membrane fission mechanism that mediates regulated endocytosis of many G protein-coupled receptors. In addition, dynamin is required for ligand-induced activation of mitogen- activated protein kinase by certain receptors, raising a general question about the role of dynamin in mitogenic signal transduction. Here we report that endocytosis of μ and δ opioid receptors is not required for efficient ligand-induced activation of mitogen-activated protein kinase. Nevertheless, mitogenic signaling mediated by these receptors is specifically dynamin- dependent. Thus a functional role of dynamin in mitogenic signaling can be dissociated from its role in receptor-mediated endocytosis, suggesting a previously unidentified and distinct role of dynamin in signal transduction by certain G protein-coupled receptors.",
author = "Jennifer Whistler and {Von Zastrow}, Mark",
year = "1999",
month = "8",
day = "27",
doi = "10.1074/jbc.274.35.24575",
language = "English (US)",
volume = "274",
pages = "24575--24578",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "35",

}

TY - JOUR

T1 - Dissociation of functional roles of dynamin in receptor-mediated endocytosis and mitogenic signal transduction

AU - Whistler, Jennifer

AU - Von Zastrow, Mark

PY - 1999/8/27

Y1 - 1999/8/27

N2 - Dynamin plays a critical role in the membrane fission mechanism that mediates regulated endocytosis of many G protein-coupled receptors. In addition, dynamin is required for ligand-induced activation of mitogen- activated protein kinase by certain receptors, raising a general question about the role of dynamin in mitogenic signal transduction. Here we report that endocytosis of μ and δ opioid receptors is not required for efficient ligand-induced activation of mitogen-activated protein kinase. Nevertheless, mitogenic signaling mediated by these receptors is specifically dynamin- dependent. Thus a functional role of dynamin in mitogenic signaling can be dissociated from its role in receptor-mediated endocytosis, suggesting a previously unidentified and distinct role of dynamin in signal transduction by certain G protein-coupled receptors.

AB - Dynamin plays a critical role in the membrane fission mechanism that mediates regulated endocytosis of many G protein-coupled receptors. In addition, dynamin is required for ligand-induced activation of mitogen- activated protein kinase by certain receptors, raising a general question about the role of dynamin in mitogenic signal transduction. Here we report that endocytosis of μ and δ opioid receptors is not required for efficient ligand-induced activation of mitogen-activated protein kinase. Nevertheless, mitogenic signaling mediated by these receptors is specifically dynamin- dependent. Thus a functional role of dynamin in mitogenic signaling can be dissociated from its role in receptor-mediated endocytosis, suggesting a previously unidentified and distinct role of dynamin in signal transduction by certain G protein-coupled receptors.

UR - http://www.scopus.com/inward/record.url?scp=0033609894&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033609894&partnerID=8YFLogxK

U2 - 10.1074/jbc.274.35.24575

DO - 10.1074/jbc.274.35.24575

M3 - Article

C2 - 10455121

AN - SCOPUS:0033609894

VL - 274

SP - 24575

EP - 24578

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 35

ER -