Dissociation between rat hippocampal CA1 and dentate gyrus cells in their response to corticosterone: Effects on calcium channel protein and current

Neeltje G. Van Gemert, Diana M M Carvalho, Henk Karst, Siem Van Der Laan, Mingxu Zhang, Onno C. Meijer, Johannes W Hell, Marian Joëls

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Stress and corticosterone affect, via glucocorticoid receptors, cellular physiology in the rodent brain. A well-documented example concerns corticosteroid effects on high-voltage activated (L type) calcium currents in the hippocampal CA1 area.Wetested whether corticosterone also affects calcium currents in another hippocampal area that highly expresses glucocorticoid receptors, i.e. the dentate gyrus (DG). Remarkably, corticosterone (100 nM, given for 20 min, 1-4.5 hr before recording) did not change high-voltage activated calcium currents in the DG, where as currents in the CA1 area of the same rats were increased. Follow-up studies revealed that no apparent dissociation between the two areas was observed with respect to transcriptional regulation of calcium channel subunits; thus, in both areas corticosterone increased mRNA levels of the calcium channel-β4 but not the (α) Cav1.2 subunit. At the protein level, however, β4 and Ca v1.2 levels were significantly up-regulated by corticosterone in the CA1 but not the DG area. These data suggest that stress-induced elevations in the level of corticosterone result in a regionally differentiated physiological response that is not simply determined by the glucocorticoid receptor distribution and that the observed regional differentiation may be caused by a gene involved in the translational machinery or in mechanisms regulating mRNA or protein stability.

Original languageEnglish (US)
Pages (from-to)4615-4624
Number of pages10
JournalEndocrinology
Volume150
Issue number10
DOIs
StatePublished - Oct 2009
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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