Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes

P. W. Roelvink, M. M M Van Meer, C. A D De Kort, R. D. Possee, B. D. Hammock, J. M. Vlak

Research output: Contribution to journalArticle

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Abstract

The temporal expression of the Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes in Spodoptera frugiperda cells was studied using virus recombinants in which either gene was replaced by the juvenile hormone esterase (JHE) gene of Heliothis virescens. The JHE served as a highly specific and sensitive reporter for gene expression. Activation of the p10 gene followed a pattern different to that of polyhedrin. The p10 gene was activated a few hours earlier than the polyhedrin gene, but its expression reached a lower maximum level. Northern blot analysis complemented and confirmed the results obtained from the JHE assays. Co-infection of sense recombinants and those containing an antisense copy of the JHE gene in place of the polyhedrin or p10 gene resulted in reduced levels of JHE gene expression. These experiments independently supported the hypothesis that the p10 gene promoter is more active at earlier times post-infection than that of the polyhedrin gene. The results also highlight the potential of the antisense strategy as an experimental approach for the study of baculovirus gene regulation and possibly insect metabolism.

Original languageEnglish (US)
Pages (from-to)1481-1489
Number of pages9
JournalJournal of General Virology
Volume73
Issue number6
StatePublished - 1992
Externally publishedYes

Fingerprint

Nucleopolyhedrovirus
Nucleocapsid
Genes
Gene Expression
Spodoptera
Baculoviridae
Coinfection
Reporter Genes
Northern Blotting
Transcriptional Activation
Insects
juvenile hormone esterase
Viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Roelvink, P. W., Van Meer, M. M. M., De Kort, C. A. D., Possee, R. D., Hammock, B. D., & Vlak, J. M. (1992). Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes. Journal of General Virology, 73(6), 1481-1489.

Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes. / Roelvink, P. W.; Van Meer, M. M M; De Kort, C. A D; Possee, R. D.; Hammock, B. D.; Vlak, J. M.

In: Journal of General Virology, Vol. 73, No. 6, 1992, p. 1481-1489.

Research output: Contribution to journalArticle

Roelvink, PW, Van Meer, MMM, De Kort, CAD, Possee, RD, Hammock, BD & Vlak, JM 1992, 'Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes', Journal of General Virology, vol. 73, no. 6, pp. 1481-1489.
Roelvink, P. W. ; Van Meer, M. M M ; De Kort, C. A D ; Possee, R. D. ; Hammock, B. D. ; Vlak, J. M. / Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes. In: Journal of General Virology. 1992 ; Vol. 73, No. 6. pp. 1481-1489.
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