Dissemination of persistent intestinal bacteria via the mesenteric lymph nodes causes typhoid relapse

Amanda J. Griffin, Lin Xi Li, Sabrina Voedisch, Oliver Pabst, Stephen J Mcsorley

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Enteric pathogens can cause relapsing infections in a proportion of treated patients, but greater understanding of this phenomenon is hindered by the lack of appropriate animal models. We report here a robust animal model of relapsing primary typhoid that initiates after apparently successful antibiotic treatment of susceptible mice. Four days of enrofloxacin treatment were sufficient to reduce bacterial loads below detectable levels in all major organs, and mice appeared otherwise healthy. However, any interruption of further antibiotic therapy allowed renewed fecal shedding and renewed bacterial growth in systemic tissues to occur, and mice eventually succumbed to relapsing infection. In vivo imaging of luminescent Salmonella identified the mesenteric lymph nodes (MLNs) as a major reservoir of relapsing infection. A magnetic-bead enrichment strategy isolated MLN-resident CD11b+ Gr-1- monocytes associated with low numbers of persistent Salmonella. However, the removal of MLNs increased the severity of typhoid relapse, demonstrating that this organ serves as a protective filter to restrain the dissemination of bacteria during antibiotic therapy. Together, these data describe a robust animal model of typhoid relapse and identify an important intestinal phagocyte subset involved in protection against the systemic spread of enteric infection.

Original languageEnglish (US)
Pages (from-to)1479-1488
Number of pages10
JournalInfection and Immunity
Volume79
Issue number4
DOIs
StatePublished - Apr 2011
Externally publishedYes

Fingerprint

Typhoid Fever
Lymph Nodes
Bacteria
Recurrence
Animal Models
Anti-Bacterial Agents
Infection
Salmonella
Bacterial Shedding
Bacterial Load
Therapeutics
Phagocytes
Monocytes
Growth

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Dissemination of persistent intestinal bacteria via the mesenteric lymph nodes causes typhoid relapse. / Griffin, Amanda J.; Li, Lin Xi; Voedisch, Sabrina; Pabst, Oliver; Mcsorley, Stephen J.

In: Infection and Immunity, Vol. 79, No. 4, 04.2011, p. 1479-1488.

Research output: Contribution to journalArticle

Griffin, Amanda J. ; Li, Lin Xi ; Voedisch, Sabrina ; Pabst, Oliver ; Mcsorley, Stephen J. / Dissemination of persistent intestinal bacteria via the mesenteric lymph nodes causes typhoid relapse. In: Infection and Immunity. 2011 ; Vol. 79, No. 4. pp. 1479-1488.
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