Disseminated Blastomyces dermatitidis, genetic group 2, infection in an alpaca (Vicugna pacos)

Denise Imai, Nikole McGreevey, Jennifer L. Anderson, Jennifer K. Meece

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A 7-year-old, female Huacaya alpaca presented with a 3-week history of left-sided head tilt and mild ataxia. Despite aggressive supportive therapy with empirical antibiotic, anti-inflammatory, thiamine, and anthelminthic therapy, neurologic signs progressed to a right-sided head tilt, positional nystagmus, and episodes of severe ataxia and obtundation. On necropsy, discrete expansile to poorly delineated infiltrative masses with tan, creamy to grumous exudate were observed in the right petrous temporal bone, right middle and inner ear, right cerebellum and medulla as well as in lung, kidney, liver, spleen, lymph nodes, and the gastrointestinal tract. Impression cytology and histology revealed a myriad of intralesional yeast, 10-25 μm in diameter, with double-contoured walls, granular protoplasm, and broad-based budding. Culture characteristics, including conidial morphology and temperature-dependent yeast phase transformation, were consistent with Blastomyces sp. Positive Blastomyces antigenuria was identified on a postmortem sample of urine. Microsatellite typing and sequencing of the internal transcribed spacer region 2 identified the infecting strain as a genetic group 2 isolate of Blastomyces dermatitidis. The present report of camelid blastomycosis adds intracranial blastomycosis to the differential list for camelid neurologic disease in endemic regions.

Original languageEnglish (US)
Pages (from-to)442-447
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume26
Issue number3
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Blastomyces
Blastomyces dermatitidis
blastomycosis
New World Camelids
Camelidae
alpacas
Blastomycosis
Ataxia
yeasts
therapeutics
nervous system diseases
cerebellum
phase transition
Yeasts
Infection
thiamin
Head
nervous system
Physiologic Nystagmus
Petrous Bone

Keywords

  • Alpacas
  • Blastomyces dermatitidis
  • camelids
  • meningoencephalitis
  • systemic mycosis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Disseminated Blastomyces dermatitidis, genetic group 2, infection in an alpaca (Vicugna pacos). / Imai, Denise; McGreevey, Nikole; Anderson, Jennifer L.; Meece, Jennifer K.

In: Journal of Veterinary Diagnostic Investigation, Vol. 26, No. 3, 01.01.2014, p. 442-447.

Research output: Contribution to journalArticle

Imai, Denise ; McGreevey, Nikole ; Anderson, Jennifer L. ; Meece, Jennifer K. / Disseminated Blastomyces dermatitidis, genetic group 2, infection in an alpaca (Vicugna pacos). In: Journal of Veterinary Diagnostic Investigation. 2014 ; Vol. 26, No. 3. pp. 442-447.
@article{da452d963b6c492d8ec9738231bcb0e9,
title = "Disseminated Blastomyces dermatitidis, genetic group 2, infection in an alpaca (Vicugna pacos)",
abstract = "A 7-year-old, female Huacaya alpaca presented with a 3-week history of left-sided head tilt and mild ataxia. Despite aggressive supportive therapy with empirical antibiotic, anti-inflammatory, thiamine, and anthelminthic therapy, neurologic signs progressed to a right-sided head tilt, positional nystagmus, and episodes of severe ataxia and obtundation. On necropsy, discrete expansile to poorly delineated infiltrative masses with tan, creamy to grumous exudate were observed in the right petrous temporal bone, right middle and inner ear, right cerebellum and medulla as well as in lung, kidney, liver, spleen, lymph nodes, and the gastrointestinal tract. Impression cytology and histology revealed a myriad of intralesional yeast, 10-25 μm in diameter, with double-contoured walls, granular protoplasm, and broad-based budding. Culture characteristics, including conidial morphology and temperature-dependent yeast phase transformation, were consistent with Blastomyces sp. Positive Blastomyces antigenuria was identified on a postmortem sample of urine. Microsatellite typing and sequencing of the internal transcribed spacer region 2 identified the infecting strain as a genetic group 2 isolate of Blastomyces dermatitidis. The present report of camelid blastomycosis adds intracranial blastomycosis to the differential list for camelid neurologic disease in endemic regions.",
keywords = "Alpacas, Blastomyces dermatitidis, camelids, meningoencephalitis, systemic mycosis",
author = "Denise Imai and Nikole McGreevey and Anderson, {Jennifer L.} and Meece, {Jennifer K.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1177/1040638714523773",
language = "English (US)",
volume = "26",
pages = "442--447",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "3",

}

TY - JOUR

T1 - Disseminated Blastomyces dermatitidis, genetic group 2, infection in an alpaca (Vicugna pacos)

AU - Imai, Denise

AU - McGreevey, Nikole

AU - Anderson, Jennifer L.

AU - Meece, Jennifer K.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - A 7-year-old, female Huacaya alpaca presented with a 3-week history of left-sided head tilt and mild ataxia. Despite aggressive supportive therapy with empirical antibiotic, anti-inflammatory, thiamine, and anthelminthic therapy, neurologic signs progressed to a right-sided head tilt, positional nystagmus, and episodes of severe ataxia and obtundation. On necropsy, discrete expansile to poorly delineated infiltrative masses with tan, creamy to grumous exudate were observed in the right petrous temporal bone, right middle and inner ear, right cerebellum and medulla as well as in lung, kidney, liver, spleen, lymph nodes, and the gastrointestinal tract. Impression cytology and histology revealed a myriad of intralesional yeast, 10-25 μm in diameter, with double-contoured walls, granular protoplasm, and broad-based budding. Culture characteristics, including conidial morphology and temperature-dependent yeast phase transformation, were consistent with Blastomyces sp. Positive Blastomyces antigenuria was identified on a postmortem sample of urine. Microsatellite typing and sequencing of the internal transcribed spacer region 2 identified the infecting strain as a genetic group 2 isolate of Blastomyces dermatitidis. The present report of camelid blastomycosis adds intracranial blastomycosis to the differential list for camelid neurologic disease in endemic regions.

AB - A 7-year-old, female Huacaya alpaca presented with a 3-week history of left-sided head tilt and mild ataxia. Despite aggressive supportive therapy with empirical antibiotic, anti-inflammatory, thiamine, and anthelminthic therapy, neurologic signs progressed to a right-sided head tilt, positional nystagmus, and episodes of severe ataxia and obtundation. On necropsy, discrete expansile to poorly delineated infiltrative masses with tan, creamy to grumous exudate were observed in the right petrous temporal bone, right middle and inner ear, right cerebellum and medulla as well as in lung, kidney, liver, spleen, lymph nodes, and the gastrointestinal tract. Impression cytology and histology revealed a myriad of intralesional yeast, 10-25 μm in diameter, with double-contoured walls, granular protoplasm, and broad-based budding. Culture characteristics, including conidial morphology and temperature-dependent yeast phase transformation, were consistent with Blastomyces sp. Positive Blastomyces antigenuria was identified on a postmortem sample of urine. Microsatellite typing and sequencing of the internal transcribed spacer region 2 identified the infecting strain as a genetic group 2 isolate of Blastomyces dermatitidis. The present report of camelid blastomycosis adds intracranial blastomycosis to the differential list for camelid neurologic disease in endemic regions.

KW - Alpacas

KW - Blastomyces dermatitidis

KW - camelids

KW - meningoencephalitis

KW - systemic mycosis

UR - http://www.scopus.com/inward/record.url?scp=84901323704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901323704&partnerID=8YFLogxK

U2 - 10.1177/1040638714523773

DO - 10.1177/1040638714523773

M3 - Article

VL - 26

SP - 442

EP - 447

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 3

ER -