Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa)

Marilena Christopoulou, Leah K. McHale, Alex Kozik, Sebastian Reyes Chin Wo, Tadeusz Wroblewski, Richard W Michelmore

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Of the over 50 phenotypic resistance genes mapped in lettuce, 25 colocalize to three major resistance clusters (MRC) on chromosomes 1, 2, and 4. Similarly, the majority of candidate resistance genes encoding nucleotide binding-leucine rich repeat (NLR) proteins genetically colocalize with phenotypic resistance loci. MRC1 and MRC4 span over 66 and 63 Mb containing 84 and 21 NLR-encoding genes, respectively, as well as 765 and 627 genes that are not related to NLR genes. Forward and reverse genetic approaches were applied to dissect MRC1 and MRC4. Transgenic lines exhibiting silencing were selected using silencing of β-glucuronidase as a reporter. Silencing of two of five NLR-encoding gene families resulted in abrogation of nine of 14 tested resistance phenotypes mapping to these two regions. At MRC1, members of the coiled coil-NLR-encoding RGC1 gene family were implicated in host and nonhost resistance through requirement for Dm5/8- and Dm45-mediated resistance to downy mildew caused by Bremia lactucae as well as the hypersensitive response to effectors AvrB, AvrRpm1, and AvrRpt2 of the nonpathogen Pseudomonas syringae. At MRC4, RGC12 family members, which encode toll interleukin receptor-NLR proteins, were implicated in Dm4-,Dm7-, Dm11-, and Dm44-mediated resistance to B. lactucae. Lesions were identified in the sequence of a candidate gene within dm7 loss-of-resistance mutant lines, confirming that RGC12G confers Dm7.

Original languageEnglish (US)
Pages (from-to)751-765
Number of pages15
JournalMolecular Plant-Microbe Interactions
Volume28
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

Lettuce
Lactuca sativa
Multigene Family
lettuce
Dissection
Genes
Bremia lactucae
genes
Interleukin Receptors
Pseudomonas syringae
Reverse Genetics
downy mildew
hypersensitive response
Chromosomes, Human, Pair 4
interleukins
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1
Glucuronidase
leucine
proteins

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Christopoulou, M., McHale, L. K., Kozik, A., Wo, S. R. C., Wroblewski, T., & Michelmore, R. W. (2015). Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa). Molecular Plant-Microbe Interactions, 28(7), 751-765. https://doi.org/10.1094/MPMI-06-14-0175-R

Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa). / Christopoulou, Marilena; McHale, Leah K.; Kozik, Alex; Wo, Sebastian Reyes Chin; Wroblewski, Tadeusz; Michelmore, Richard W.

In: Molecular Plant-Microbe Interactions, Vol. 28, No. 7, 01.07.2015, p. 751-765.

Research output: Contribution to journalArticle

Christopoulou, M, McHale, LK, Kozik, A, Wo, SRC, Wroblewski, T & Michelmore, RW 2015, 'Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa)', Molecular Plant-Microbe Interactions, vol. 28, no. 7, pp. 751-765. https://doi.org/10.1094/MPMI-06-14-0175-R
Christopoulou, Marilena ; McHale, Leah K. ; Kozik, Alex ; Wo, Sebastian Reyes Chin ; Wroblewski, Tadeusz ; Michelmore, Richard W. / Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa). In: Molecular Plant-Microbe Interactions. 2015 ; Vol. 28, No. 7. pp. 751-765.
@article{b5e4bb93a0c74d4f9de9ce86389bf246,
title = "Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa)",
abstract = "Of the over 50 phenotypic resistance genes mapped in lettuce, 25 colocalize to three major resistance clusters (MRC) on chromosomes 1, 2, and 4. Similarly, the majority of candidate resistance genes encoding nucleotide binding-leucine rich repeat (NLR) proteins genetically colocalize with phenotypic resistance loci. MRC1 and MRC4 span over 66 and 63 Mb containing 84 and 21 NLR-encoding genes, respectively, as well as 765 and 627 genes that are not related to NLR genes. Forward and reverse genetic approaches were applied to dissect MRC1 and MRC4. Transgenic lines exhibiting silencing were selected using silencing of β-glucuronidase as a reporter. Silencing of two of five NLR-encoding gene families resulted in abrogation of nine of 14 tested resistance phenotypes mapping to these two regions. At MRC1, members of the coiled coil-NLR-encoding RGC1 gene family were implicated in host and nonhost resistance through requirement for Dm5/8- and Dm45-mediated resistance to downy mildew caused by Bremia lactucae as well as the hypersensitive response to effectors AvrB, AvrRpm1, and AvrRpt2 of the nonpathogen Pseudomonas syringae. At MRC4, RGC12 family members, which encode toll interleukin receptor-NLR proteins, were implicated in Dm4-,Dm7-, Dm11-, and Dm44-mediated resistance to B. lactucae. Lesions were identified in the sequence of a candidate gene within dm7 loss-of-resistance mutant lines, confirming that RGC12G confers Dm7.",
author = "Marilena Christopoulou and McHale, {Leah K.} and Alex Kozik and Wo, {Sebastian Reyes Chin} and Tadeusz Wroblewski and Michelmore, {Richard W}",
year = "2015",
month = "7",
day = "1",
doi = "10.1094/MPMI-06-14-0175-R",
language = "English (US)",
volume = "28",
pages = "751--765",
journal = "Molecular Plant-Microbe Interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "7",

}

TY - JOUR

T1 - Dissection of two complex clusters of resistance genes in lettuce (Lactuca sativa)

AU - Christopoulou, Marilena

AU - McHale, Leah K.

AU - Kozik, Alex

AU - Wo, Sebastian Reyes Chin

AU - Wroblewski, Tadeusz

AU - Michelmore, Richard W

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Of the over 50 phenotypic resistance genes mapped in lettuce, 25 colocalize to three major resistance clusters (MRC) on chromosomes 1, 2, and 4. Similarly, the majority of candidate resistance genes encoding nucleotide binding-leucine rich repeat (NLR) proteins genetically colocalize with phenotypic resistance loci. MRC1 and MRC4 span over 66 and 63 Mb containing 84 and 21 NLR-encoding genes, respectively, as well as 765 and 627 genes that are not related to NLR genes. Forward and reverse genetic approaches were applied to dissect MRC1 and MRC4. Transgenic lines exhibiting silencing were selected using silencing of β-glucuronidase as a reporter. Silencing of two of five NLR-encoding gene families resulted in abrogation of nine of 14 tested resistance phenotypes mapping to these two regions. At MRC1, members of the coiled coil-NLR-encoding RGC1 gene family were implicated in host and nonhost resistance through requirement for Dm5/8- and Dm45-mediated resistance to downy mildew caused by Bremia lactucae as well as the hypersensitive response to effectors AvrB, AvrRpm1, and AvrRpt2 of the nonpathogen Pseudomonas syringae. At MRC4, RGC12 family members, which encode toll interleukin receptor-NLR proteins, were implicated in Dm4-,Dm7-, Dm11-, and Dm44-mediated resistance to B. lactucae. Lesions were identified in the sequence of a candidate gene within dm7 loss-of-resistance mutant lines, confirming that RGC12G confers Dm7.

AB - Of the over 50 phenotypic resistance genes mapped in lettuce, 25 colocalize to three major resistance clusters (MRC) on chromosomes 1, 2, and 4. Similarly, the majority of candidate resistance genes encoding nucleotide binding-leucine rich repeat (NLR) proteins genetically colocalize with phenotypic resistance loci. MRC1 and MRC4 span over 66 and 63 Mb containing 84 and 21 NLR-encoding genes, respectively, as well as 765 and 627 genes that are not related to NLR genes. Forward and reverse genetic approaches were applied to dissect MRC1 and MRC4. Transgenic lines exhibiting silencing were selected using silencing of β-glucuronidase as a reporter. Silencing of two of five NLR-encoding gene families resulted in abrogation of nine of 14 tested resistance phenotypes mapping to these two regions. At MRC1, members of the coiled coil-NLR-encoding RGC1 gene family were implicated in host and nonhost resistance through requirement for Dm5/8- and Dm45-mediated resistance to downy mildew caused by Bremia lactucae as well as the hypersensitive response to effectors AvrB, AvrRpm1, and AvrRpt2 of the nonpathogen Pseudomonas syringae. At MRC4, RGC12 family members, which encode toll interleukin receptor-NLR proteins, were implicated in Dm4-,Dm7-, Dm11-, and Dm44-mediated resistance to B. lactucae. Lesions were identified in the sequence of a candidate gene within dm7 loss-of-resistance mutant lines, confirming that RGC12G confers Dm7.

UR - http://www.scopus.com/inward/record.url?scp=84938237595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938237595&partnerID=8YFLogxK

U2 - 10.1094/MPMI-06-14-0175-R

DO - 10.1094/MPMI-06-14-0175-R

M3 - Article

C2 - 25650829

AN - SCOPUS:84938237595

VL - 28

SP - 751

EP - 765

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

SN - 0894-0282

IS - 7

ER -