Disruption of steroid and prolactin receptor patterning in the mammary gland correlates with a block in lobuloalveolar development

Sandra L. Grimm, Tiffany N. Seagroves, Elena B. Kabotyanski, Russell C. Hovey, Barbara K. Vonderhaar, John P. Lydon, Keiko Miyoshi, Lothar Hennighausen, Christopher J. Ormandy, Adrian V. Lee, Malinda A. Stull, Teresa L. Wood, Jeffrey M. Rosen

Research output: Contribution to journalArticle

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Abstract

Targeted deletion of the bZIP transcription factor, CCAAT/enhancer binding protein-β (C/EBPβ), was shown previously to result in aberrant ductal morphogenesis and decreased lobuloalveolar development, accompanied by an altered pattern of progesterone receptor (PR) expression. Here, similar changes in the level and pattern of prolactin receptor (PrIR) expression were observed while screening for differentially expressed genes in C/EBPβnull mice. PR patterning was also altered in PrIRnull mice, as well as in mammary tissue transplants from both PrIRnull and signal transducer and activator of transcription (Stat) 5a/b-deficient mice, with concomitant defects in hormone-induced proliferation. Down-regulation of PR and activation of Stat5 phosphorylation were seen after estrogen and progesterone treatment in both C/EBPβnull and wild-type mice, indicating that these signaling pathways were functional, despite the failure of steroid hormones to induce proliferation. IGF binding protein-5, IGF-II, and insulin receptor substrate-1 all displayed altered patterns and levels of expression in C/EBPβnull mice, suggestive of a change in the IGF signaling axis. In addition, small proline-rich protein (SPRR2A), a marker of epidermal differentiation, and keratin 6 were misexpressed in the mammary epithelium of C/EBPβnull mice. Together, these data suggest that C/EBPβ is a master regulator of mammary epithelial cell fate and that the correct spatial pattern of PR and PrIR expression is a critical determinant of hormone-regulated cell proliferation.

Original languageEnglish (US)
Pages (from-to)2675-2691
Number of pages17
JournalMolecular Endocrinology
Volume16
Issue number12
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Prolactin Receptors
Steroid Receptors
Human Mammary Glands
Progesterone Receptors
CCAAT-Enhancer-Binding Proteins
Breast
Hormones
Keratin-6
Insulin-Like Growth Factor Binding Protein 5
Basic-Leucine Zipper Transcription Factors
STAT5 Transcription Factor
IGF Type 2 Receptor
Insulin Receptor Substrate Proteins
Differentiation Antigens
Morphogenesis
Proline
Progesterone
Estrogens
Down-Regulation
Epithelium

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Grimm, S. L., Seagroves, T. N., Kabotyanski, E. B., Hovey, R. C., Vonderhaar, B. K., Lydon, J. P., ... Rosen, J. M. (2002). Disruption of steroid and prolactin receptor patterning in the mammary gland correlates with a block in lobuloalveolar development. Molecular Endocrinology, 16(12), 2675-2691. https://doi.org/10.1210/me.2002-0239

Disruption of steroid and prolactin receptor patterning in the mammary gland correlates with a block in lobuloalveolar development. / Grimm, Sandra L.; Seagroves, Tiffany N.; Kabotyanski, Elena B.; Hovey, Russell C.; Vonderhaar, Barbara K.; Lydon, John P.; Miyoshi, Keiko; Hennighausen, Lothar; Ormandy, Christopher J.; Lee, Adrian V.; Stull, Malinda A.; Wood, Teresa L.; Rosen, Jeffrey M.

In: Molecular Endocrinology, Vol. 16, No. 12, 01.12.2002, p. 2675-2691.

Research output: Contribution to journalArticle

Grimm, SL, Seagroves, TN, Kabotyanski, EB, Hovey, RC, Vonderhaar, BK, Lydon, JP, Miyoshi, K, Hennighausen, L, Ormandy, CJ, Lee, AV, Stull, MA, Wood, TL & Rosen, JM 2002, 'Disruption of steroid and prolactin receptor patterning in the mammary gland correlates with a block in lobuloalveolar development', Molecular Endocrinology, vol. 16, no. 12, pp. 2675-2691. https://doi.org/10.1210/me.2002-0239
Grimm, Sandra L. ; Seagroves, Tiffany N. ; Kabotyanski, Elena B. ; Hovey, Russell C. ; Vonderhaar, Barbara K. ; Lydon, John P. ; Miyoshi, Keiko ; Hennighausen, Lothar ; Ormandy, Christopher J. ; Lee, Adrian V. ; Stull, Malinda A. ; Wood, Teresa L. ; Rosen, Jeffrey M. / Disruption of steroid and prolactin receptor patterning in the mammary gland correlates with a block in lobuloalveolar development. In: Molecular Endocrinology. 2002 ; Vol. 16, No. 12. pp. 2675-2691.
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AU - Vonderhaar, Barbara K.

AU - Lydon, John P.

AU - Miyoshi, Keiko

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AU - Ormandy, Christopher J.

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AU - Stull, Malinda A.

AU - Wood, Teresa L.

AU - Rosen, Jeffrey M.

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