Disruption of mitochondrial function as mechanism for anti-cancer activity of a novel mitochondriotropic menadione derivative

José Teixeira, Ricardo Amorim, Katia Santos, Pedro Soares, Sandipan Datta, Gino A Cortopassi, Teresa L. Serafim, Vilma A. Sardão, Jorge Garrido, Fernanda Borges, Paulo J. Oliveira

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Menadione, also known as vitamin K3, is a 2-methyl-1,4 naphthoquinone with a potent cytotoxic activity mainly resulting from its quinone redox-cycling with production of reactive oxygen species (ROS). Although increased ROS generation is considered a relevant mechanism in cancer cell death, it may not be sufficiently effective to kill cancer cells due to phenotypic adaptations. Therefore, combining ROS-generating agents with other molecules targeting important cancer cell phenotypes can be an effective therapeutic strategy. As mitochondrial dysfunction has been implicated in many human diseases, including cancer, we describe here the discovery of a mitochondrial-directed agent (MitoK3), which was developed by conjugating a TPP cation to the C3 position of the menadione's naphthoquinone ring, increasing its selective accumulation in mitochondria, as well as led to alterations of its redox properties and consequent biological outcome. MitoK3 disturbed the mitochondrial bioenergetic apparatus, with subsequent loss of mitochondrial ATP production. The combinatory strategy of MitoK3 with anticancer agent doxorubicin (DOX) resulted in a degree of cytotoxicity higher than those of the individual molecules, as the combination triggered tumour apoptotic cell death evident by caspase 3/9 activities, probably through mitochondrial destabilization or by interference with mitochondrial redox processes. The results of this investigation support the importance of drug discovery process in developing molecules that can be use as adjuvant therapy in patients with specific cancer subtypes.

Original languageEnglish (US)
Pages (from-to)123-139
Number of pages17
JournalToxicology
Volume393
DOIs
StatePublished - Jan 15 2018

Keywords

  • Doxorubicin
  • Drug discovery
  • Menadione
  • Mitochondria
  • Mitochondriotropic menadione derivative

ASJC Scopus subject areas

  • Toxicology

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    Teixeira, J., Amorim, R., Santos, K., Soares, P., Datta, S., Cortopassi, G. A., Serafim, T. L., Sardão, V. A., Garrido, J., Borges, F., & Oliveira, P. J. (2018). Disruption of mitochondrial function as mechanism for anti-cancer activity of a novel mitochondriotropic menadione derivative. Toxicology, 393, 123-139. https://doi.org/10.1016/j.tox.2017.11.014