Abstract
Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 628-632 |
| Number of pages | 5 |
| Journal | Experimental Cell Research |
| Volume | 173 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs. / Nishioka, David; Porter, Donald C.; Trimmer, James; Vacquier, Victor D.
In: Experimental Cell Research, Vol. 173, No. 2, 1987, p. 628-632.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs
AU - Nishioka, David
AU - Porter, Donald C.
AU - Trimmer, James
AU - Vacquier, Victor D.
PY - 1987
Y1 - 1987
N2 - Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.
AB - Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.
UR - http://www.scopus.com/inward/record.url?scp=0023609453&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023609453&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(87)90301-6
DO - 10.1016/0014-4827(87)90301-6
M3 - Article
VL - 173
SP - 628
EP - 632
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -