Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs

David Nishioka; Donald C. Porter; James S. Trimmer; Victor D. Vacquier

doi:10.1016/0014-4827(87)90301-6

Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs

David Nishioka, Donald C. Porter, James S. Trimmer, Victor D. Vacquier

Neurobiology, Physiology and Behavior

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.

Original language	English (US)
Pages (from-to)	628-632
Number of pages	5
Journal	Experimental Cell Research
Volume	173
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(87)90301-6
State	Published - 1987

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(87)90301-6

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title = "Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs",

abstract = "Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.",

author = "David Nishioka and Porter, {Donald C.} and Trimmer, {James S.} and Vacquier, {Victor D.}",

year = "1987",

doi = "10.1016/0014-4827(87)90301-6",

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journal = "Experimental Cell Research",

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T1 - Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs

AU - Nishioka, David

AU - Porter, Donald C.

AU - Trimmer, James S.

AU - Vacquier, Victor D.

PY - 1987

Y1 - 1987

N2 - Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.

AB - Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.

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Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs

Abstract

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