Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs

David Nishioka, Donald C. Porter, James Trimmer, Victor D. Vacquier

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Polyspermically fertilized Strongylocentrotus purpuratus eggs were fixed at varying times after insemination and exposed to a monoclonal antibody (mAb J18/29) directed against a group of sperm surface antigens. Indirect immunofluorescence microscopy reveals that the sperm surface components recognized by mAb J18/29 are quickly incorporated into the egg plasma membrane and begin to disperse as early as 1.5 min after insemination. At subsequent times after insemination, they undergo further dispersal so that by 45 min they are distributed evenly over the entire surface of the egg. These results provide evidence for the free lateral mobility of sperm membrane components in the fertilized egg.

Original languageEnglish (US)
Pages (from-to)628-632
Number of pages5
JournalExperimental Cell Research
Volume173
Issue number2
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Sea Urchins
Insemination
Surface Antigens
Eggs
Spermatozoa
Cell Membrane
Ovum
Strongylocentrotus purpuratus
Zygote
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Monoclonal Antibodies
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs. / Nishioka, David; Porter, Donald C.; Trimmer, James; Vacquier, Victor D.

In: Experimental Cell Research, Vol. 173, No. 2, 1987, p. 628-632.

Research output: Contribution to journalArticle

Nishioka, David ; Porter, Donald C. ; Trimmer, James ; Vacquier, Victor D. / Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs. In: Experimental Cell Research. 1987 ; Vol. 173, No. 2. pp. 628-632.
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