Disease mutations in RUNX1 and RUNX2 create nonfunctional, dominant-negative, or hypomorphic alleles

Christina J. Matheny, Maren E. Speck, Patrick R. Cushing, Yunpeng Zhou, Takeshi Corpora, Michael Regan, Miki Newman, Liya Roudaia, Caroline L. Speck, Ting Lei Gu, Stephen M Griffey, John H. Bushweller, Nancy A. Speck

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Monoallelic RUNX1 mutations cause familial platelet disorder with predisposition for acute myelogenous leukemia (FPD/AML). Sporadic mono- and biallelic mutations are found at high frequencies in AML M0, in radiation-associated and therapy-related myelodysplastic syndrome and AML, and in isolated cases of AML M2, M5a, M3 relapse, and chronic myelogenous leukemia in blast phase. Mutations in RUNX2 cause the inherited skeletal disorder cleidocranial dysplasia (CCD). Most hematopoietic missense mutations in Runx1 involve DNA-contacting residues in the Runt domain, whereas the majority of CCD mutations in Runx2 are predicted to impair CBFβ binding or the Runt domain structure. We introduced different classes of missense mutations into Runx1 and characterized their effects on DNA and CBFβ binding by the Runt domain, and on Runx1 function in vivo. Mutations involving DNA-contacting residues severely inactivate Runx1 function, whereas mutations that affect CBFβ binding but not DNA binding result in hypomorphic alleles. We conclude that hypomorphic RUNX2 alleles can cause CCD, whereas hematopoietic disease requires more severely inactivating RUNX1 mutations.

Original languageEnglish (US)
Pages (from-to)1163-1175
Number of pages13
JournalEMBO Journal
Volume26
Issue number4
DOIs
StatePublished - Feb 21 2007

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Keywords

  • Cleidocranial dysplasia
  • Core binding factors
  • Leukemia
  • Runx1
  • Runx2

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Matheny, C. J., Speck, M. E., Cushing, P. R., Zhou, Y., Corpora, T., Regan, M., Newman, M., Roudaia, L., Speck, C. L., Gu, T. L., Griffey, S. M., Bushweller, J. H., & Speck, N. A. (2007). Disease mutations in RUNX1 and RUNX2 create nonfunctional, dominant-negative, or hypomorphic alleles. EMBO Journal, 26(4), 1163-1175. https://doi.org/10.1038/sj.emboj.7601568