Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy

Laura Marcu, Michael C. Fishbein, Jean Michel I Maarek, Warren S. Grundfest

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.

Original languageEnglish (US)
Pages (from-to)1244-1250
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume21
Issue number7
StatePublished - 2001

Fingerprint

Fluorescence Spectrometry
Coronary Vessels
Lasers
Lipids
Fluorescence
Gas Lasers
Rupture

Keywords

  • Atherosclerosis
  • Lesion instability
  • Spectroscopy
  • Time-resolved laser-induced fluorescence

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy. / Marcu, Laura; Fishbein, Michael C.; Maarek, Jean Michel I; Grundfest, Warren S.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 21, No. 7, 2001, p. 1244-1250.

Research output: Contribution to journalArticle

Marcu, Laura ; Fishbein, Michael C. ; Maarek, Jean Michel I ; Grundfest, Warren S. / Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2001 ; Vol. 21, No. 7. pp. 1244-1250.
@article{6f8afdc2a40647ebb9c007eb33bf3145,
title = "Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy",
abstract = "Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.",
keywords = "Atherosclerosis, Lesion instability, Spectroscopy, Time-resolved laser-induced fluorescence",
author = "Laura Marcu and Fishbein, {Michael C.} and Maarek, {Jean Michel I} and Grundfest, {Warren S.}",
year = "2001",
language = "English (US)",
volume = "21",
pages = "1244--1250",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy

AU - Marcu, Laura

AU - Fishbein, Michael C.

AU - Maarek, Jean Michel I

AU - Grundfest, Warren S.

PY - 2001

Y1 - 2001

N2 - Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.

AB - Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.

KW - Atherosclerosis

KW - Lesion instability

KW - Spectroscopy

KW - Time-resolved laser-induced fluorescence

UR - http://www.scopus.com/inward/record.url?scp=0035720530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035720530&partnerID=8YFLogxK

M3 - Article

C2 - 11451759

AN - SCOPUS:0035720530

VL - 21

SP - 1244

EP - 1250

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 7

ER -