Abstract
Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1244-1250 |
| Number of pages | 7 |
| Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
| Volume | 21 |
| Issue number | 7 |
| State | Published - 2001 |
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Keywords
- Atherosclerosis
- Lesion instability
- Spectroscopy
- Time-resolved laser-induced fluorescence
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy. / Marcu, Laura; Fishbein, Michael C.; Maarek, Jean Michel I; Grundfest, Warren S.
In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 21, No. 7, 2001, p. 1244-1250.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy
AU - Marcu, Laura
AU - Fishbein, Michael C.
AU - Maarek, Jean Michel I
AU - Grundfest, Warren S.
PY - 2001
Y1 - 2001
N2 - Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.
AB - Lesion composition plays a significant role in atherosclerotic lesion instability and rupture. Current clinical techniques cannot fully characterize lesion composition or accurately identify unstable lesions. This study investigates the use of time-resolved fluorescence spectroscopy for unstable atherosclerotic lesion diagnosis. The fluorescence of human coronary artery samples was induced with nitrogen laser and detected in the 360- to 510-nm wavelength range. The samples were sorted into 7 groups according to the AHA classification: normal wall and types I, IIa (fatty streaks), III (preatheroma), IV (atheroma), Va (fibrous), and Vb (calcified) lesions. Spectral intensities and time-dependent parameters [average lifetime τf; decay constants: τ1 (fast-term), τ2 (slow-term), A1 (fast-term amplitude contribution)] derived from the time-resolved spectra of coronary samples were used for tissue characterization. We determined that a few intensity values at longer wavelengths (>430 nm) and time-dependent parameters at peak emission region (390 nm) discriminate between all types of arterial samples except between normal wall and type I lesions. The lipid-rich lesions (more unstable) can be discriminated from fibrous lesions (more stable) on the basis of time-dependent parameters (lifetime and fast-term decay). We inferred that features of lipid fluorescence are reflected on lipid-rich lesion emission. Our results demonstrate that analysis of the time-resolved spectra may be used to enhance the discrimination between different grades of atherosclerotic lesions and provide a means of discrimination between lipid-rich and fibrous lesions.
KW - Atherosclerosis
KW - Lesion instability
KW - Spectroscopy
KW - Time-resolved laser-induced fluorescence
UR - http://www.scopus.com/inward/record.url?scp=0035720530&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035720530&partnerID=8YFLogxK
M3 - Article
C2 - 11451759
AN - SCOPUS:0035720530
VL - 21
SP - 1244
EP - 1250
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 7
ER -