Abstract
An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.
Original language | English (US) |
---|---|
Pages (from-to) | 2938-2942 |
Number of pages | 5 |
Journal | Bioorganic and Medicinal Chemistry Letters |
Volume | 15 |
Issue number | 11 |
DOIs | |
State | Published - Jun 2 2005 |
Keywords
- ALR1
- ALR2
- On-bead assay
- Protein binding
- Protein fluorescent labeling
- Selective binding
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Drug Discovery
- Pharmaceutical Science