Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy

Seth Dixon, Lori Robins, Robert A. Elling, Ruiwu Liu, Kit Lam, David K. Wilson, Mark J. Kurth

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.

Original languageEnglish (US)
Pages (from-to)2938-2942
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume15
Issue number11
DOIs
StatePublished - Jun 2 2005

Keywords

  • ALR1
  • ALR2
  • On-bead assay
  • Protein binding
  • Protein fluorescent labeling
  • Selective binding

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

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