Abstract
An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2938-2942 |
| Number of pages | 5 |
| Journal | Bioorganic and Medicinal Chemistry Letters |
| Volume | 15 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 2 2005 |
Keywords
- ALR1
- ALR2
- On-bead assay
- Protein binding
- Protein fluorescent labeling
- Selective binding
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Drug Discovery
- Pharmaceutical Science
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Dixon, S., Robins, L., Elling, R. A., Liu, R., Lam, K., Wilson, D. K., & Kurth, M. J. (2005). Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy. Bioorganic and Medicinal Chemistry Letters, 15(11), 2938-2942. https://doi.org/10.1016/j.bmcl.2005.03.010