Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy

Seth Dixon; Lori Robins; Robert A. Elling; Ruiwu Liu; Kit Lam; David K. Wilson; Mark J. Kurth

doi:10.1016/j.bmcl.2005.03.010

Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy

Seth Dixon, Lori Robins, Robert A. Elling, Ruiwu Liu, Kit Lam, David K. Wilson, Mark J. Kurth

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.

Original language	English (US)
Pages (from-to)	2938-2942
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	15
Issue number	11
DOIs	https://doi.org/10.1016/j.bmcl.2005.03.010
State	Published - Jun 2 2005

Keywords

ALR1
ALR2
On-bead assay
Protein binding
Protein fluorescent labeling
Selective binding

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Organic Chemistry
Drug Discovery
Pharmaceutical Science

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10.1016/j.bmcl.2005.03.010

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title = "Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy",

abstract = "An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.",

keywords = "ALR1, ALR2, On-bead assay, Protein binding, Protein fluorescent labeling, Selective binding",

author = "Seth Dixon and Lori Robins and Elling, {Robert A.} and Ruiwu Liu and Kit Lam and Wilson, {David K.} and Kurth, {Mark J.}",

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language = "English (US)",

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T1 - Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy

AU - Dixon, Seth

AU - Robins, Lori

AU - Elling, Robert A.

AU - Liu, Ruiwu

AU - Lam, Kit

AU - Wilson, David K.

AU - Kurth, Mark J.

PY - 2005/6/2

Y1 - 2005/6/2

N2 - An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.

AB - An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.

KW - ALR1

KW - ALR2

KW - On-bead assay

KW - Protein binding

KW - Protein fluorescent labeling

KW - Selective binding

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DO - 10.1016/j.bmcl.2005.03.010

M3 - Article

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AN - SCOPUS:19544371932

VL - 15

SP - 2938

EP - 2942

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

IS - 11

ER -

Discovery of selective aldo-keto reductase ligands - An on-bead assay strategy

Abstract

Keywords

ASJC Scopus subject areas

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