Discovery of laryngeal carcinoma by serum proteomic pattern analysis

Xueyuan Xiao, Xiaodong Zhao, Jiankai Liu, Fuzheng Guo, Danhui Liu, Dacheng He

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Laryngeal carcinoma is the most common malignancy among head and neck tumors. The purpose of this study is to find biomarkers for laryngeal carcinoma in patient blood serum using the Surface Enhanced Laser Desorption/lonization (SELDI) technique. Serum samples from 33 laryngeal carcinoma (12 cases of glottis, 18 of supraglottis and 3 of subglottis) patients and 31 age- and sex-matched healthy people were analyzed by SELDI-TOF on a ProteinChip reader, PBSII-C. Protein profiles were generated using WCX2 protein chips. Protein peak clustering and classification analyses were performed utilizing the Biomarker Wizard and Biomarker Pattern software packages, respectively. The results showed that sixteen peaks had significant difference between laryngeal cancer patients and healthy group, eight of which were up-regulated in the patient samples, and the others were down-regulated. Two protein peaks 8153 Da and 2035 Da were automatically chosen for the system training and development of a classification tree. The analysis yielded a correct percentage of 96.9% for patients and 96.7% for control. The results suggest that serum is a useful resource for the detection of specific biomarkers for laryngeal carcinoma. Proteinchip Array System was a useful tool for a high throughput screening of large-sized serum samples to discover potential biomarkers for carcinoma. Copyright by Science in China Press 2004.

Original languageEnglish (US)
Pages (from-to)219-223
Number of pages5
JournalScience in China, Series C: Life Sciences
Volume47
Issue number3
DOIs
StatePublished - Dec 1 2004
Externally publishedYes

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Keywords

  • Biomarker pattern
  • Laryngeal carcinoma
  • Protein chip array system

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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