Discovery of genes implicated in whirling disease infection and resistance in rainbow trout using genome-wide expression profiling

Melinda R. Baerwald, Amy B. Welsh, Ronald Hedrick, Bernie May

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background: Whirling disease, caused by the pathogen Myxobolus cerebralis, afflicts several salmonid species. Rainbow trout are particularly susceptible and may suffer high mortality rates. The disease is persistent and spreading in hatcheries and natural waters of several countries, including the U.S.A., and the economic losses attributed to whirling disease are substantial. In this study, genome-wide expression profiling using cDNA microarrays was conducted for resistant Hofer and susceptible Trout Lodge rainbow trout strains following pathogen exposure with the primary objective of identifying specific genes implicated in whirling disease resistance. Results: Several genes were significantly up-regulated in skin following pathogen exposure for both the resistant and susceptible rainbow trout strains. For both strains, response to infection appears to be linked with the interferon system. Expression profiles for three genes identified with microarrays were confirmed with qRT-PCR. Ubiquitin-like protein 1 was up-regulated over 100 fold and interferon regulating factor 1 was up-regulated over 15 fold following pathogen exposure for both strains. Expression of metallothionein B, which has known roles in inflammation and immune response, was up-regulated over 5 fold in the resistant Hofer strain but was unchanged in the susceptible Trout Lodge strain following pathogen exposure. Conclusion: The present study has provided an initial view into the genetic basis underlying immune response and resistance of rainbow trout to the whirling disease parasite. The identified genes have allowed us to gain insight into the molecular mechanisms implicated in salmonid immune response and resistance to whirling disease infection.

Original languageEnglish (US)
Article number37
JournalBMC Genomics
Volume9
DOIs
StatePublished - Jan 24 2008

Fingerprint

Disease Resistance
Oncorhynchus mykiss
Genetic Association Studies
Genome
Infection
Trout
Interferons
Myxobolus
Ubiquitins
Genes
Metallothionein
Oligonucleotide Array Sequence Analysis
Transcriptome
Parasites
Economics
Inflammation
Polymerase Chain Reaction
Skin
Mortality
Water

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Discovery of genes implicated in whirling disease infection and resistance in rainbow trout using genome-wide expression profiling. / Baerwald, Melinda R.; Welsh, Amy B.; Hedrick, Ronald; May, Bernie.

In: BMC Genomics, Vol. 9, 37, 24.01.2008.

Research output: Contribution to journalArticle

@article{4e7933083c684518be1d0e8105616a83,
title = "Discovery of genes implicated in whirling disease infection and resistance in rainbow trout using genome-wide expression profiling",
abstract = "Background: Whirling disease, caused by the pathogen Myxobolus cerebralis, afflicts several salmonid species. Rainbow trout are particularly susceptible and may suffer high mortality rates. The disease is persistent and spreading in hatcheries and natural waters of several countries, including the U.S.A., and the economic losses attributed to whirling disease are substantial. In this study, genome-wide expression profiling using cDNA microarrays was conducted for resistant Hofer and susceptible Trout Lodge rainbow trout strains following pathogen exposure with the primary objective of identifying specific genes implicated in whirling disease resistance. Results: Several genes were significantly up-regulated in skin following pathogen exposure for both the resistant and susceptible rainbow trout strains. For both strains, response to infection appears to be linked with the interferon system. Expression profiles for three genes identified with microarrays were confirmed with qRT-PCR. Ubiquitin-like protein 1 was up-regulated over 100 fold and interferon regulating factor 1 was up-regulated over 15 fold following pathogen exposure for both strains. Expression of metallothionein B, which has known roles in inflammation and immune response, was up-regulated over 5 fold in the resistant Hofer strain but was unchanged in the susceptible Trout Lodge strain following pathogen exposure. Conclusion: The present study has provided an initial view into the genetic basis underlying immune response and resistance of rainbow trout to the whirling disease parasite. The identified genes have allowed us to gain insight into the molecular mechanisms implicated in salmonid immune response and resistance to whirling disease infection.",
author = "Baerwald, {Melinda R.} and Welsh, {Amy B.} and Ronald Hedrick and Bernie May",
year = "2008",
month = "1",
day = "24",
doi = "10.1186/1471-2164-9-37",
language = "English (US)",
volume = "9",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Discovery of genes implicated in whirling disease infection and resistance in rainbow trout using genome-wide expression profiling

AU - Baerwald, Melinda R.

AU - Welsh, Amy B.

AU - Hedrick, Ronald

AU - May, Bernie

PY - 2008/1/24

Y1 - 2008/1/24

N2 - Background: Whirling disease, caused by the pathogen Myxobolus cerebralis, afflicts several salmonid species. Rainbow trout are particularly susceptible and may suffer high mortality rates. The disease is persistent and spreading in hatcheries and natural waters of several countries, including the U.S.A., and the economic losses attributed to whirling disease are substantial. In this study, genome-wide expression profiling using cDNA microarrays was conducted for resistant Hofer and susceptible Trout Lodge rainbow trout strains following pathogen exposure with the primary objective of identifying specific genes implicated in whirling disease resistance. Results: Several genes were significantly up-regulated in skin following pathogen exposure for both the resistant and susceptible rainbow trout strains. For both strains, response to infection appears to be linked with the interferon system. Expression profiles for three genes identified with microarrays were confirmed with qRT-PCR. Ubiquitin-like protein 1 was up-regulated over 100 fold and interferon regulating factor 1 was up-regulated over 15 fold following pathogen exposure for both strains. Expression of metallothionein B, which has known roles in inflammation and immune response, was up-regulated over 5 fold in the resistant Hofer strain but was unchanged in the susceptible Trout Lodge strain following pathogen exposure. Conclusion: The present study has provided an initial view into the genetic basis underlying immune response and resistance of rainbow trout to the whirling disease parasite. The identified genes have allowed us to gain insight into the molecular mechanisms implicated in salmonid immune response and resistance to whirling disease infection.

AB - Background: Whirling disease, caused by the pathogen Myxobolus cerebralis, afflicts several salmonid species. Rainbow trout are particularly susceptible and may suffer high mortality rates. The disease is persistent and spreading in hatcheries and natural waters of several countries, including the U.S.A., and the economic losses attributed to whirling disease are substantial. In this study, genome-wide expression profiling using cDNA microarrays was conducted for resistant Hofer and susceptible Trout Lodge rainbow trout strains following pathogen exposure with the primary objective of identifying specific genes implicated in whirling disease resistance. Results: Several genes were significantly up-regulated in skin following pathogen exposure for both the resistant and susceptible rainbow trout strains. For both strains, response to infection appears to be linked with the interferon system. Expression profiles for three genes identified with microarrays were confirmed with qRT-PCR. Ubiquitin-like protein 1 was up-regulated over 100 fold and interferon regulating factor 1 was up-regulated over 15 fold following pathogen exposure for both strains. Expression of metallothionein B, which has known roles in inflammation and immune response, was up-regulated over 5 fold in the resistant Hofer strain but was unchanged in the susceptible Trout Lodge strain following pathogen exposure. Conclusion: The present study has provided an initial view into the genetic basis underlying immune response and resistance of rainbow trout to the whirling disease parasite. The identified genes have allowed us to gain insight into the molecular mechanisms implicated in salmonid immune response and resistance to whirling disease infection.

UR - http://www.scopus.com/inward/record.url?scp=39849099078&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39849099078&partnerID=8YFLogxK

U2 - 10.1186/1471-2164-9-37

DO - 10.1186/1471-2164-9-37

M3 - Article

C2 - 18218127

AN - SCOPUS:39849099078

VL - 9

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 37

ER -