Discovery of D-amino-acidcontaining ligands with selectide technology

Kit Lam, Michal Lebl, Victor Krchnak, Shelly Wade, Farid Abdul-Latif, Ronald Ferguson, Christine Cuzzocrea, Ken Wertman

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

When all L-amino acid (aa) random peptide libraries synthesized on solid-phase particles were screened (Selectide Technology), we identified several peptide ligands (YG_F_) that interacted specifically with an anti-β-endorphin monoclonal antibody (clone 3E7) (single-letter aa symbols; symbols '_' indicate variable aa). Here, we report on the screening of three different D-aa-containing pentapeptide libraries (XxXxX, xXxXx and xxxxx, wherein X = L-aa, and X = D-aa) with the same antibody, in which several D-aa-containing ligands were identified. The binding affinities of many of these D-aa-containing ligands were as least two orders of magnitude lower than that of YGGFL, for which the Ki is 17.5 nM.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalGene
Volume137
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • Antibody
  • Mimotope
  • Streptavidin
  • Synthetic peptide library

ASJC Scopus subject areas

  • Genetics

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