Discovery of D-amino-acidcontaining ligands with selectide technology

Kit Lam, Michal Lebl, Victor Krchnak, Shelly Wade, Farid Abdul-Latif, Ronald Ferguson, Christine Cuzzocrea, Ken Wertman

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

When all L-amino acid (aa) random peptide libraries synthesized on solid-phase particles were screened (Selectide Technology), we identified several peptide ligands (YG_F_) that interacted specifically with an anti-β-endorphin monoclonal antibody (clone 3E7) (single-letter aa symbols; symbols '_' indicate variable aa). Here, we report on the screening of three different D-aa-containing pentapeptide libraries (XxXxX, xXxXx and xxxxx, wherein X = L-aa, and X = D-aa) with the same antibody, in which several D-aa-containing ligands were identified. The binding affinities of many of these D-aa-containing ligands were as least two orders of magnitude lower than that of YGGFL, for which the Ki is 17.5 nM.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalGene
Volume137
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Ligands
Technology
Amino Acids
Endorphins
Peptide Library
Libraries
Clone Cells
Monoclonal Antibodies
Peptides
Antibodies

Keywords

  • Antibody
  • Mimotope
  • Streptavidin
  • Synthetic peptide library

ASJC Scopus subject areas

  • Genetics

Cite this

Lam, K., Lebl, M., Krchnak, V., Wade, S., Abdul-Latif, F., Ferguson, R., ... Wertman, K. (1993). Discovery of D-amino-acidcontaining ligands with selectide technology. Gene, 137(1), 19-24. https://doi.org/10.1016/0378-1119(93)90245-X

Discovery of D-amino-acidcontaining ligands with selectide technology. / Lam, Kit; Lebl, Michal; Krchnak, Victor; Wade, Shelly; Abdul-Latif, Farid; Ferguson, Ronald; Cuzzocrea, Christine; Wertman, Ken.

In: Gene, Vol. 137, No. 1, 1993, p. 19-24.

Research output: Contribution to journalArticle

Lam, K, Lebl, M, Krchnak, V, Wade, S, Abdul-Latif, F, Ferguson, R, Cuzzocrea, C & Wertman, K 1993, 'Discovery of D-amino-acidcontaining ligands with selectide technology', Gene, vol. 137, no. 1, pp. 19-24. https://doi.org/10.1016/0378-1119(93)90245-X
Lam K, Lebl M, Krchnak V, Wade S, Abdul-Latif F, Ferguson R et al. Discovery of D-amino-acidcontaining ligands with selectide technology. Gene. 1993;137(1):19-24. https://doi.org/10.1016/0378-1119(93)90245-X
Lam, Kit ; Lebl, Michal ; Krchnak, Victor ; Wade, Shelly ; Abdul-Latif, Farid ; Ferguson, Ronald ; Cuzzocrea, Christine ; Wertman, Ken. / Discovery of D-amino-acidcontaining ligands with selectide technology. In: Gene. 1993 ; Vol. 137, No. 1. pp. 19-24.
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