Directed evolution of trypsin inhibiting peptides using a genetic algorithm

Yohei Yokobayashi, Kazunori Ikebukuro, Scott McNiven, Isao Karube

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A new strategy which employs a process of directed evolution in the search for molecules exhibiting certain desirable properties is reported. By repeating a cycle of peptide synthesis, evaluation of the trypsin inhibitory activities of these peptides and subsequent selection and transformation based on a genetic algorithm, it is possible artificially to induce the evolution of a family of peptides and improve their biological activities. Commencing with a set of 24 randomly generated hexapeptides, a progressive improvement from 16 to 50% average inhibitory activity over six generations, with maximum activities of 80-90% is observed. The emergence of consensus sequences which concur with those previously generated using peptide libraries is also observed.

Original languageEnglish (US)
Pages (from-to)2435-2437
Number of pages3
JournalJournal of the Chemical Society - Perkin Transactions 1
Issue number20
StatePublished - 1996
Externally publishedYes

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Trypsin
Genetic algorithms
Peptides
Peptide Library
Bioactivity
Molecules

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Yokobayashi, Y., Ikebukuro, K., McNiven, S., & Karube, I. (1996). Directed evolution of trypsin inhibiting peptides using a genetic algorithm. Journal of the Chemical Society - Perkin Transactions 1, (20), 2435-2437.

Directed evolution of trypsin inhibiting peptides using a genetic algorithm. / Yokobayashi, Yohei; Ikebukuro, Kazunori; McNiven, Scott; Karube, Isao.

In: Journal of the Chemical Society - Perkin Transactions 1, No. 20, 1996, p. 2435-2437.

Research output: Contribution to journalArticle

Yokobayashi, Y, Ikebukuro, K, McNiven, S & Karube, I 1996, 'Directed evolution of trypsin inhibiting peptides using a genetic algorithm', Journal of the Chemical Society - Perkin Transactions 1, no. 20, pp. 2435-2437.
Yokobayashi, Yohei ; Ikebukuro, Kazunori ; McNiven, Scott ; Karube, Isao. / Directed evolution of trypsin inhibiting peptides using a genetic algorithm. In: Journal of the Chemical Society - Perkin Transactions 1. 1996 ; No. 20. pp. 2435-2437.
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