Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development

Minelva R. Nanton, Seung Joo Lee, Shaikh M. Atif, Sean Paul Nuccio, Justin J. Taylor, Andreas J Baumler, Sing Sing Way, Stephen J Mcsorley

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

CD4+ T cells and B cells are both essential for acquired immunity to Salmonella infection. It is well established that Salmonella inhibit host CD4+ T-cell responses, but a corresponding inhibitory effect on B cells is less well defined. Here, we utilize an Ag tetramer and pull-down enrichment strategy to directly visualize OVA-specific B cells in mice, as they respond to infection with Salmonella-OVA. Surprisingly, OVA-specific B-cell expansion and germinal center formation was not detected until bacteria were cleared from the host. Furthermore, Salmonella infection also actively inhibited both B- and T-cell responses to the same coinjected Ag but this did not require the presence of iNOS. The Salmonella Pathogenicity Island 2 (SPI2) locus has been shown to be responsible for inhibition of Salmonella-specific CD4+ T-cell responses, and an examination of SPI2-deficient bacteria demonstrated a recovery in B-cell expansion in infected mice. Together, these data suggest that Salmonella can simultaneously inhibit host B- and T-cell responses using SPI2-dependent mechanisms.

Original languageEnglish (US)
Pages (from-to)428-441
Number of pages14
JournalEuropean Journal of Immunology
Volume45
Issue number2
DOIs
StatePublished - Feb 1 2015

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Keywords

  • B cells
  • Bacterial infection
  • Clonal expansion
  • Germinal centers
  • Immunity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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