Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development

Minelva R. Nanton, Seung Joo Lee, Shaikh M. Atif, Sean Paul Nuccio, Justin J. Taylor, Andreas J Baumler, Sing Sing Way, Stephen J Mcsorley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

CD4+ T cells and B cells are both essential for acquired immunity to Salmonella infection. It is well established that Salmonella inhibit host CD4+ T-cell responses, but a corresponding inhibitory effect on B cells is less well defined. Here, we utilize an Ag tetramer and pull-down enrichment strategy to directly visualize OVA-specific B cells in mice, as they respond to infection with Salmonella-OVA. Surprisingly, OVA-specific B-cell expansion and germinal center formation was not detected until bacteria were cleared from the host. Furthermore, Salmonella infection also actively inhibited both B- and T-cell responses to the same coinjected Ag but this did not require the presence of iNOS. The Salmonella Pathogenicity Island 2 (SPI2) locus has been shown to be responsible for inhibition of Salmonella-specific CD4+ T-cell responses, and an examination of SPI2-deficient bacteria demonstrated a recovery in B-cell expansion in infected mice. Together, these data suggest that Salmonella can simultaneously inhibit host B- and T-cell responses using SPI2-dependent mechanisms.

Original languageEnglish (US)
Pages (from-to)428-441
Number of pages14
JournalEuropean Journal of Immunology
Volume45
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Germinal Center
Salmonella
B-Lymphocytes
Genomic Islands
Salmonella Infections
T-Lymphocytes
Bacteria
Adaptive Immunity

Keywords

  • B cells
  • Bacterial infection
  • Clonal expansion
  • Germinal centers
  • Immunity

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development. / Nanton, Minelva R.; Lee, Seung Joo; Atif, Shaikh M.; Nuccio, Sean Paul; Taylor, Justin J.; Baumler, Andreas J; Way, Sing Sing; Mcsorley, Stephen J.

In: European Journal of Immunology, Vol. 45, No. 2, 01.02.2015, p. 428-441.

Research output: Contribution to journalArticle

Nanton, Minelva R. ; Lee, Seung Joo ; Atif, Shaikh M. ; Nuccio, Sean Paul ; Taylor, Justin J. ; Baumler, Andreas J ; Way, Sing Sing ; Mcsorley, Stephen J. / Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development. In: European Journal of Immunology. 2015 ; Vol. 45, No. 2. pp. 428-441.
@article{308ba35f077c4068ba17b491c54959f0,
title = "Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development",
abstract = "CD4+ T cells and B cells are both essential for acquired immunity to Salmonella infection. It is well established that Salmonella inhibit host CD4+ T-cell responses, but a corresponding inhibitory effect on B cells is less well defined. Here, we utilize an Ag tetramer and pull-down enrichment strategy to directly visualize OVA-specific B cells in mice, as they respond to infection with Salmonella-OVA. Surprisingly, OVA-specific B-cell expansion and germinal center formation was not detected until bacteria were cleared from the host. Furthermore, Salmonella infection also actively inhibited both B- and T-cell responses to the same coinjected Ag but this did not require the presence of iNOS. The Salmonella Pathogenicity Island 2 (SPI2) locus has been shown to be responsible for inhibition of Salmonella-specific CD4+ T-cell responses, and an examination of SPI2-deficient bacteria demonstrated a recovery in B-cell expansion in infected mice. Together, these data suggest that Salmonella can simultaneously inhibit host B- and T-cell responses using SPI2-dependent mechanisms.",
keywords = "B cells, Bacterial infection, Clonal expansion, Germinal centers, Immunity",
author = "Nanton, {Minelva R.} and Lee, {Seung Joo} and Atif, {Shaikh M.} and Nuccio, {Sean Paul} and Taylor, {Justin J.} and Baumler, {Andreas J} and Way, {Sing Sing} and Mcsorley, {Stephen J}",
year = "2015",
month = "2",
day = "1",
doi = "10.1002/eji.201444540",
language = "English (US)",
volume = "45",
pages = "428--441",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "2",

}

TY - JOUR

T1 - Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development

AU - Nanton, Minelva R.

AU - Lee, Seung Joo

AU - Atif, Shaikh M.

AU - Nuccio, Sean Paul

AU - Taylor, Justin J.

AU - Baumler, Andreas J

AU - Way, Sing Sing

AU - Mcsorley, Stephen J

PY - 2015/2/1

Y1 - 2015/2/1

N2 - CD4+ T cells and B cells are both essential for acquired immunity to Salmonella infection. It is well established that Salmonella inhibit host CD4+ T-cell responses, but a corresponding inhibitory effect on B cells is less well defined. Here, we utilize an Ag tetramer and pull-down enrichment strategy to directly visualize OVA-specific B cells in mice, as they respond to infection with Salmonella-OVA. Surprisingly, OVA-specific B-cell expansion and germinal center formation was not detected until bacteria were cleared from the host. Furthermore, Salmonella infection also actively inhibited both B- and T-cell responses to the same coinjected Ag but this did not require the presence of iNOS. The Salmonella Pathogenicity Island 2 (SPI2) locus has been shown to be responsible for inhibition of Salmonella-specific CD4+ T-cell responses, and an examination of SPI2-deficient bacteria demonstrated a recovery in B-cell expansion in infected mice. Together, these data suggest that Salmonella can simultaneously inhibit host B- and T-cell responses using SPI2-dependent mechanisms.

AB - CD4+ T cells and B cells are both essential for acquired immunity to Salmonella infection. It is well established that Salmonella inhibit host CD4+ T-cell responses, but a corresponding inhibitory effect on B cells is less well defined. Here, we utilize an Ag tetramer and pull-down enrichment strategy to directly visualize OVA-specific B cells in mice, as they respond to infection with Salmonella-OVA. Surprisingly, OVA-specific B-cell expansion and germinal center formation was not detected until bacteria were cleared from the host. Furthermore, Salmonella infection also actively inhibited both B- and T-cell responses to the same coinjected Ag but this did not require the presence of iNOS. The Salmonella Pathogenicity Island 2 (SPI2) locus has been shown to be responsible for inhibition of Salmonella-specific CD4+ T-cell responses, and an examination of SPI2-deficient bacteria demonstrated a recovery in B-cell expansion in infected mice. Together, these data suggest that Salmonella can simultaneously inhibit host B- and T-cell responses using SPI2-dependent mechanisms.

KW - B cells

KW - Bacterial infection

KW - Clonal expansion

KW - Germinal centers

KW - Immunity

UR - http://www.scopus.com/inward/record.url?scp=84923011623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923011623&partnerID=8YFLogxK

U2 - 10.1002/eji.201444540

DO - 10.1002/eji.201444540

M3 - Article

C2 - 25346524

AN - SCOPUS:84923011623

VL - 45

SP - 428

EP - 441

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -