Abstract
A wet photolithographic route for micropatterning fluid phospholipid bilayers is demonstrated in which spatially directed illumination by short-wavelength ultraviolet radiation results in highly localized photochemical degradation of the exposed lipids. Using this method, we can directly engineer patterns of hydrophilic voids within a fluid membrane as well as isolated membrane corrals over large substrate areas. We show that the lipid-free regions can be refilled by the same or other lipids and lipid mixtures which establish contiguity with the existing membrane, thereby providing a synthetic means for manipulating membrane compositions, engineering metastable membrane microdomains, probing 2D lipid-lipid mixing, and designing membrane-embedded arrays of soluble proteins. Following this route, new constructs can be envisaged for high-throughput membrane proteomic, biosensor array, and spatially directed, aqueous- phase material synthesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 13962-13972 |
| Number of pages | 11 |
| Journal | Journal of the American Chemical Society |
| Volume | 126 |
| Issue number | 43 |
| DOIs | |
| State | Published - Nov 3 2004 |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
Direct photochemical patterning and refunctionalization of supported phospholipid bilayers. / Yee, Chanel K.; Amweg, Meri L.; Parikh, Atul N.
In: Journal of the American Chemical Society, Vol. 126, No. 43, 03.11.2004, p. 13962-13972.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct photochemical patterning and refunctionalization of supported phospholipid bilayers
AU - Yee, Chanel K.
AU - Amweg, Meri L.
AU - Parikh, Atul N.
PY - 2004/11/3
Y1 - 2004/11/3
N2 - A wet photolithographic route for micropatterning fluid phospholipid bilayers is demonstrated in which spatially directed illumination by short-wavelength ultraviolet radiation results in highly localized photochemical degradation of the exposed lipids. Using this method, we can directly engineer patterns of hydrophilic voids within a fluid membrane as well as isolated membrane corrals over large substrate areas. We show that the lipid-free regions can be refilled by the same or other lipids and lipid mixtures which establish contiguity with the existing membrane, thereby providing a synthetic means for manipulating membrane compositions, engineering metastable membrane microdomains, probing 2D lipid-lipid mixing, and designing membrane-embedded arrays of soluble proteins. Following this route, new constructs can be envisaged for high-throughput membrane proteomic, biosensor array, and spatially directed, aqueous- phase material synthesis.
AB - A wet photolithographic route for micropatterning fluid phospholipid bilayers is demonstrated in which spatially directed illumination by short-wavelength ultraviolet radiation results in highly localized photochemical degradation of the exposed lipids. Using this method, we can directly engineer patterns of hydrophilic voids within a fluid membrane as well as isolated membrane corrals over large substrate areas. We show that the lipid-free regions can be refilled by the same or other lipids and lipid mixtures which establish contiguity with the existing membrane, thereby providing a synthetic means for manipulating membrane compositions, engineering metastable membrane microdomains, probing 2D lipid-lipid mixing, and designing membrane-embedded arrays of soluble proteins. Following this route, new constructs can be envisaged for high-throughput membrane proteomic, biosensor array, and spatially directed, aqueous- phase material synthesis.
UR - http://www.scopus.com/inward/record.url?scp=7444253461&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7444253461&partnerID=8YFLogxK
U2 - 10.1021/ja047714k
DO - 10.1021/ja047714k
M3 - Article
C2 - 15506757
AN - SCOPUS:7444253461
VL - 126
SP - 13962
EP - 13972
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 43
ER -