Direct observations of ultrasound microbubble contrast agent interaction with the microvessel wall

Charles F. Caskey, Susanne M. Stieger, Shengping Qin, Paul A. Dayton, Katherine W. Ferrara

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Many thousands of contrast ultrasound studies have been conducted in clinics around the world. In addition, the microbubbles employed in these examinations are being widely investigated to deliver drugs and genes. Here, for the first time, the oscillation of these microbubbles in small vessels is directly observed and shown to be substantially different than that predicted by previous models and imaged within large fluid volumes. Using pulsed ultrasound with a center frequency of 1 MHz and peak rarefactional pressure of 0.8 or 2.0 MPa, microbubble expansion was significantly reduced when microbubbles were constrained within small vessels in the rat cecum (p<0.05). A model for microbubble oscillation within compliant vessels is presented that accurately predicts oscillation and vessel displacement within small vessels. As a result of the decreased oscillation in small vessels, a large resting microbubble diameter resulting from agent fusion or a high mechanical index was required to bring the agent shell into contact with the endothelium. Also, contact with the endothelium was observed during asymmetrical collapse, not during expansion. These results will be used to improve the design of drug delivery techniques using microbubbles.

Original languageEnglish (US)
Pages (from-to)1191-1200
Number of pages10
JournalJournal of the Acoustical Society of America
Volume122
Issue number2
DOIs
StatePublished - 2007

Fingerprint

vessels
endothelium
oscillations
interactions
drugs
expansion
genes
rats
Ultrasound
Vessel
Interaction
delivery
examination
fusion
Oscillation
fluids
Drugs

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

Cite this

Direct observations of ultrasound microbubble contrast agent interaction with the microvessel wall. / Caskey, Charles F.; Stieger, Susanne M.; Qin, Shengping; Dayton, Paul A.; Ferrara, Katherine W.

In: Journal of the Acoustical Society of America, Vol. 122, No. 2, 2007, p. 1191-1200.

Research output: Contribution to journalArticle

Caskey, Charles F. ; Stieger, Susanne M. ; Qin, Shengping ; Dayton, Paul A. ; Ferrara, Katherine W. / Direct observations of ultrasound microbubble contrast agent interaction with the microvessel wall. In: Journal of the Acoustical Society of America. 2007 ; Vol. 122, No. 2. pp. 1191-1200.
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