TY - JOUR
T1 - Direct observations of silver nanowire-induced frustrated phagocytosis among NR8383 lung alveolar macrophages
AU - Ogorodnik, Evgeny
AU - Karsai, Arpad
AU - Wang, Kang Hsin
AU - Liu, Fu Tong
AU - Lo, Su Hao
AU - Pinkerton, Kent E.
AU - Gilbert, Benjamin
AU - Haudenschild, Dominik R
AU - Liu, Gang Yu
N1 - Funding Information:
We thank Ms. Victoria Tran for her technical assistance in SEM and EDS measurements. This work was supported by the UC Davis, the National Institutes of Health (R01-AR070239), the W.M. Keck Foundation, the National Science Foundation (CHE-1808829), the National Institute of Environmental Health Sciences (U01-ES027288), the Gordon and Betty Moore Foundation, and partly supported by DOE BES under Contract DE-AC02-05CH11231.
PY - 2020/12/24
Y1 - 2020/12/24
N2 - The interaction of long nanowires and living cells is directly related to nanowires' nanotoxicity and health impacts. Interactions of silver nanowires (AgNWs) and macrophage cell lines (NR8383) were investigated using laser scanning confocal microscopy and single cell compression (SCC). With high-resolution imaging and mechanics measurement of individual cells, AgNW-induced frustrated phagocytosis was clearly captured in conjunction with structural and property changes of cells. While frustrated phagocytosis is known for long microwires and long carbon nanotubes, this work reports first direct observations of frustrated phagocytosis of AgNWs among living cells in situ. In the case of partial penetration of AgNWs into NR8383 cells, confocal imaging revealed actin participation at the entry sites, whose behavior differs from microwire-induced frustrated phagocytosis. The impacts of frustrated phagocytosis on the cellular membrane and cytoskeleton were also quantified by measuring the mechanical properties using SCC. Taken collectively, this study reveals the structural and property characteristics of nanowire-induced frustrated phagocytosis, which deepens our understanding of nanowire-cell interactions and nanocytotoxicity.
AB - The interaction of long nanowires and living cells is directly related to nanowires' nanotoxicity and health impacts. Interactions of silver nanowires (AgNWs) and macrophage cell lines (NR8383) were investigated using laser scanning confocal microscopy and single cell compression (SCC). With high-resolution imaging and mechanics measurement of individual cells, AgNW-induced frustrated phagocytosis was clearly captured in conjunction with structural and property changes of cells. While frustrated phagocytosis is known for long microwires and long carbon nanotubes, this work reports first direct observations of frustrated phagocytosis of AgNWs among living cells in situ. In the case of partial penetration of AgNWs into NR8383 cells, confocal imaging revealed actin participation at the entry sites, whose behavior differs from microwire-induced frustrated phagocytosis. The impacts of frustrated phagocytosis on the cellular membrane and cytoskeleton were also quantified by measuring the mechanical properties using SCC. Taken collectively, this study reveals the structural and property characteristics of nanowire-induced frustrated phagocytosis, which deepens our understanding of nanowire-cell interactions and nanocytotoxicity.
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U2 - 10.1021/acs.jpcb.0c08132
DO - 10.1021/acs.jpcb.0c08132
M3 - Article
C2 - 33306381
AN - SCOPUS:85098780169
VL - 124
SP - 11584
EP - 11592
JO - The journal of physical chemistry. B
JF - The journal of physical chemistry. B
SN - 1520-5207
IS - 51
ER -