Direct Observation of Tunneling Nanotubes within Human Mesenchymal Stem Cell Spheroids

Jiali Zhang, Jacklyn Whitehead, Yang Liu, Qingbo Yang, Jonathan K Leach, Gang-yu Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tunneling nanotubes (TNTs) play an important role in cell-cell communication. TNTs have been predominantly reported among cells in monolayer culture. Using various imaging modalities, including scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM), this work reports the finding of TNTs between cells within human mesenchymal stem cell (MSC) spheroids. TNTs visualized by SEM are consistent in size and geometry with those observed in cellular monolayer culture. LSCM imaging of living spheroids confirms the presence of F-actin filaments within the TNTs, which are known to maintain nanotube integrity. In addition, LSCM revealed the distribution of F-actin fibers across the entire spheroid body instead of being confined within individual cells. Intracellular material transport by TNTs was tested in MSC spheroids treated with cytochalasin D (CytoD), a known actin polymerization inhibitor for disrupting TNT formation. CytoD treatment decreased the transport of cytosolic material by at least four-fold compared to untreated spheroids. To the best of our knowledge, this work represents the first direct observation of TNTs within MSC spheroids. These findings offer new physical insight into cellular interactions within spheroids, providing structural information for increasing interests in spheroid-based cell therapy.

Original languageEnglish (US)
Pages (from-to)9920-9926
Number of pages7
JournalJournal of Physical Chemistry B
Volume122
Issue number43
DOIs
StatePublished - Nov 1 2018

Fingerprint

Nanotubes
stem cells
spheroids
Stem cells
Mesenchymal Stromal Cells
nanotubes
Observation
Confocal microscopy
cells
Confocal Microscopy
Actins
Cytochalasin D
microscopy
Scanning
Electron Scanning Microscopy
scanning
Lasers
Monolayers
lasers
Imaging techniques

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Direct Observation of Tunneling Nanotubes within Human Mesenchymal Stem Cell Spheroids. / Zhang, Jiali; Whitehead, Jacklyn; Liu, Yang; Yang, Qingbo; Leach, Jonathan K; Liu, Gang-yu.

In: Journal of Physical Chemistry B, Vol. 122, No. 43, 01.11.2018, p. 9920-9926.

Research output: Contribution to journalArticle

Zhang, Jiali ; Whitehead, Jacklyn ; Liu, Yang ; Yang, Qingbo ; Leach, Jonathan K ; Liu, Gang-yu. / Direct Observation of Tunneling Nanotubes within Human Mesenchymal Stem Cell Spheroids. In: Journal of Physical Chemistry B. 2018 ; Vol. 122, No. 43. pp. 9920-9926.
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