Direct observation of the p188 mediated membrane sealing with atomic force microscopy

R. C. Lee, F. Despa, X. Tang, I. Titushkin, M. Cho

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The structural changes in fibroblast cell membranes associated with permeability increases due to a chemical injury by saponin and successive Poloxamer 188 (P188) mediated membrane sealing were characterized using atomic force microscope (AFM). In combination with the AFM imaging, a cell viability measurement was also performed. Ethidium homodimer-1 (EH) saponin/water and calcien-AM were used to stain the cells. Direct evidence was obtained that P188 restore the integrity of compromised cell membranes and increase the survival of the treated cells.

Original languageEnglish (US)
Pages (from-to)185-186
Number of pages2
JournalMCB Molecular and Cellular Biomechanics
Volume3
Issue number4
StatePublished - Dec 2006
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Biophysics
  • Molecular Biology
  • Molecular Medicine

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