Direct modulation by Ca2+-calmodulin of cyclic nucleotide-activated channel of rat olfactory receptor neurons

Tsung-Yu Chen, King Wal Yau

Research output: Contribution to journalArticlepeer-review

243 Scopus citations

Abstract

OLFACTORY receptor neurons depolarize in response to odorant stimulation of their sensory cilia1-3. One transduction mechanism involves a G-protein-mediated increase in adenylate cyclase activity4-8, raising the internal cyclic AMP concentration to open a cyclic nucleotide-activated cation channel on the plasma membrane9-14. An influx of Ca2+ through this channel, which is permeable to both monovalent and divalent cations, triggers olfactory adaptation15. Previous work has indicated that at least part of this Ca2+ -mediated adaptation resides in the channel itself15-17, but the mechanism remains unclear and controversial16-18. Here we use the cloned channel from rat19 expressed in a cell line and the native channel from rat olfactory receptor cells to show that Ca2+ reduces the apparent affinity of the channel for cAMP by up to 20-fold in the presence of calmodulin, an abundant protein in olfactory cilia20. This decrease in apparent affinity appears to involve a direct interaction between Ca2+-calmodulin and the channel, and it can reduce the activation of the channel by cAMP by up to a few hundred-fold, suggesting that it may be a key component of the Ca2+ -triggered olfactory adaptation.

Original languageEnglish (US)
Pages (from-to)545-548
Number of pages4
JournalNature
Volume368
Issue number6471
StatePublished - Apr 7 1994
Externally publishedYes

ASJC Scopus subject areas

  • General

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