Direct measurement of the lamellipodial protrusive force in a migrating cell

Marcus Prass, Ken Jacobson, Alex Mogilner, Manfred Radmacher

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

There has been a great deal of interest in the mechanism of lamellipodial protrusion (Pollard, T., and G. Borisy. 2003. Cell. 112:453-465). However, one of this mechanism's endpoints, the force of protrusion, has never been directly measured. We place an atomic force microscopy cantilever in the path of a migrating keratocyte. The deflection of the cantilever, which occurs over a period of ∼10 s, provides a direct measure of the force exerted by the lamellipodial leading edge. Stall forces are consistent with ∼100 polymerizing actin filaments per micrometer of the leading edge, each working as an elastic Brownian ratchet and generating a force of several piconewtons. However, the force-velocity curves obtained from this measurement, in which velocity drops sharply under very small loads, is not sensitive to low loading forces, and finally stalls rapidly at large loads, are not consistent with current theoretical models for the actin polymerization force. Rather, the curves indicate that the protrusive force generation is a complex multiphase process involving actin and adhesion dynamics.

Original languageEnglish (US)
Pages (from-to)767-772
Number of pages6
JournalJournal of Cell Biology
Volume174
Issue number6
DOIs
StatePublished - Sep 11 2006

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Actins
Atomic Force Microscopy
Actin Cytoskeleton
Polymerization
Theoretical Models

ASJC Scopus subject areas

  • Cell Biology

Cite this

Direct measurement of the lamellipodial protrusive force in a migrating cell. / Prass, Marcus; Jacobson, Ken; Mogilner, Alex; Radmacher, Manfred.

In: Journal of Cell Biology, Vol. 174, No. 6, 11.09.2006, p. 767-772.

Research output: Contribution to journalArticle

Prass, M, Jacobson, K, Mogilner, A & Radmacher, M 2006, 'Direct measurement of the lamellipodial protrusive force in a migrating cell', Journal of Cell Biology, vol. 174, no. 6, pp. 767-772. https://doi.org/10.1083/jcb.200601159
Prass, Marcus ; Jacobson, Ken ; Mogilner, Alex ; Radmacher, Manfred. / Direct measurement of the lamellipodial protrusive force in a migrating cell. In: Journal of Cell Biology. 2006 ; Vol. 174, No. 6. pp. 767-772.
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