Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function

Thomas J. Jaw, Li Ru You, Paul S Knoepfler, Li Chin Yao, Chi Yun Pai, Chiou Yang Tang, Li Ping Chang, Jens Berthelsen, Francesco Blasi, Mark P. Kamps, Y. Henry Sun

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH. This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)279-291
Number of pages13
JournalMechanisms of Development
Volume91
Issue number1-2
DOIs
StatePublished - Mar 1 2000

Keywords

  • Antenna
  • Drosophila
  • Extradenticle
  • Eye
  • Homothorax
  • HOX
  • Nuclear export
  • PREP1

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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    Jaw, T. J., You, L. R., Knoepfler, P. S., Yao, L. C., Pai, C. Y., Tang, C. Y., Chang, L. P., Berthelsen, J., Blasi, F., Kamps, M. P., & Sun, Y. H. (2000). Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function. Mechanisms of Development, 91(1-2), 279-291. https://doi.org/10.1016/S0925-4773(99)00316-0