Abstract
The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH. This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 279-291 |
| Number of pages | 13 |
| Journal | Mechanisms of Development |
| Volume | 91 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Mar 1 2000 |
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Keywords
- Antenna
- Drosophila
- Extradenticle
- Eye
- Homothorax
- HOX
- Nuclear export
- PREP1
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function. / Jaw, Thomas J.; You, Li Ru; Knoepfler, Paul S; Yao, Li Chin; Pai, Chi Yun; Tang, Chiou Yang; Chang, Li Ping; Berthelsen, Jens; Blasi, Francesco; Kamps, Mark P.; Sun, Y. Henry.
In: Mechanisms of Development, Vol. 91, No. 1-2, 01.03.2000, p. 279-291.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function
AU - Jaw, Thomas J.
AU - You, Li Ru
AU - Knoepfler, Paul S
AU - Yao, Li Chin
AU - Pai, Chi Yun
AU - Tang, Chiou Yang
AU - Chang, Li Ping
AU - Berthelsen, Jens
AU - Blasi, Francesco
AU - Kamps, Mark P.
AU - Sun, Y. Henry
PY - 2000/3/1
Y1 - 2000/3/1
N2 - The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH. This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd.
AB - The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH. This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd.
KW - Antenna
KW - Drosophila
KW - Extradenticle
KW - Eye
KW - Homothorax
KW - HOX
KW - Nuclear export
KW - PREP1
UR - http://www.scopus.com/inward/record.url?scp=0033993481&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033993481&partnerID=8YFLogxK
U2 - 10.1016/S0925-4773(99)00316-0
DO - 10.1016/S0925-4773(99)00316-0
M3 - Article
C2 - 10704852
AN - SCOPUS:0033993481
VL - 91
SP - 279
EP - 291
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 1-2
ER -