Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function

Thomas J. Jaw; Li Ru You; Paul S Knoepfler; Li Chin Yao; Chi Yun Pai; Chiou Yang Tang; Li Ping Chang; Jens Berthelsen; Francesco Blasi; Mark P. Kamps; Y. Henry Sun

doi:10.1016/S0925-4773(99)00316-0

Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function

Thomas J. Jaw, Li Ru You, Paul S Knoepfler, Li Chin Yao, Chi Yun Pai, Chiou Yang Tang, Li Ping Chang, Jens Berthelsen, Francesco Blasi, Mark P. Kamps, Y. Henry Sun

Cell Biology and Human Anatomy

Research output: Contribution to journal › Article

71 Scopus citations

Abstract

The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH. This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	279-291
Number of pages	13
Journal	Mechanisms of Development
Volume	91
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(99)00316-0
State	Published - Mar 1 2000

Keywords

Antenna
Drosophila
Extradenticle
Eye
Homothorax
HOX
Nuclear export
PREP1

ASJC Scopus subject areas

Developmental Biology
Developmental Neuroscience

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Jaw, T. J., You, L. R., Knoepfler, P. S., Yao, L. C., Pai, C. Y., Tang, C. Y., Chang, L. P., Berthelsen, J., Blasi, F., Kamps, M. P., & Sun, Y. H. (2000). Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function. Mechanisms of Development, 91(1-2), 279-291. https://doi.org/10.1016/S0925-4773(99)00316-0

Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function. / Jaw, Thomas J.; You, Li Ru; Knoepfler, Paul S; Yao, Li Chin; Pai, Chi Yun; Tang, Chiou Yang; Chang, Li Ping; Berthelsen, Jens; Blasi, Francesco; Kamps, Mark P.; Sun, Y. Henry.

In: Mechanisms of Development, Vol. 91, No. 1-2, 01.03.2000, p. 279-291.

Research output: Contribution to journal › Article

Jaw, Thomas J. ; You, Li Ru ; Knoepfler, Paul S ; Yao, Li Chin ; Pai, Chi Yun ; Tang, Chiou Yang ; Chang, Li Ping ; Berthelsen, Jens ; Blasi, Francesco ; Kamps, Mark P. ; Sun, Y. Henry. / Direct interaction of two homeoproteins, Homothorax and Extradenticle, is essential for EXD nuclear localization and function. In: Mechanisms of Development. 2000 ; Vol. 91, No. 1-2. pp. 279-291.

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abstract = "The Drosophila Homothorax (HTH) and Extradenticle (EXD) are two homeoproteins required in a number of developmental processes. EXD can function as a cofactor to Hox proteins. Its nuclear localization is dependent on HTH. In this study we present evidence of in vivo physical interaction between HTH and EXD, mediated primarily through an evolutionarily conserved MH domain in HTH. This interaction is essential for the mutual stabilization of both proteins, for EXD nuclear localization, and for the cooperative DNA binding of the EXD-HTH heterodimer. Some in vivo functions require both EXD and HTH in the nucleus, suggesting that the EXD-HTH complex may function as a transcriptional regulator. (C) 2000 Elsevier Science Ireland Ltd.",

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