Direct fluorescence monitoring of DNA base excision repair

Toshikazu Ono, Shenliang Wang, Chi Kin Koo, Lisa Engstrom, Sheila S. David, Eric T. Kool

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Uracil is a common form of DNA damage resulting from hydrolysis of cytosine, and cellular uracil DNA glycosylases (UDG) have evolved to remove it specifically. The use of nonnatural pyrene deoxyriboside in short DNA oligomers to directly report on UDG enzymatic activity is described. The mechanism relies on the use of uracil as a strong quencher of pyrene, and enzyme repair activity can be directly imaged with bacterial cells in real time.

Original languageEnglish (US)
Pages (from-to)1689-1692
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number7
DOIs
StatePublished - Feb 13 2012

Keywords

  • fluorescence
  • oligonucleotides
  • pyrene
  • uracil
  • uracil DNA glycosylase

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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    Ono, T., Wang, S., Koo, C. K., Engstrom, L., David, S. S., & Kool, E. T. (2012). Direct fluorescence monitoring of DNA base excision repair. Angewandte Chemie - International Edition, 51(7), 1689-1692. https://doi.org/10.1002/anie.201108135