Direct evidence for the interaction of neurokinin A with the tachykinin NK1 receptor in tissue

Andrew A. Bremer, Morris F. Tansky, Meiye Wu, Norman D. Boyd, Susan E. Leeman

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Neurokinin A (NKA) is a tachykinin peptide that binds with high affinity to the tachykinin NK2 receptor. Recent homologous binding studies, however, have shown that neurokinin A is also a high-affinity ligand for the tachykinin NK1 receptor. In this report, we demonstrate that a photoreactive neurokinin A analogue specifically labels the NK1 receptor in rat submandibular gland membranes and show via bioassay that neurokinin A is a potent stimulator of salivary secretion. Through the use of specific non-peptide antagonists in both photolabeling and functional assays, we unequivocally demonstrate that neurokinin A can specifically interact with the NK1 receptor in vivo and elicit NK1 receptor-mediated physiological responses.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalEuropean Journal of Pharmacology
Volume423
Issue number2-3
DOIs
StatePublished - Jul 6 2001
Externally publishedYes

Keywords

  • Bioassay
  • Neurokinin A
  • Photoaffinity labeling
  • Tachykinin NK receptor

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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