Abstract
Neurokinin A (NKA) is a tachykinin peptide that binds with high affinity to the tachykinin NK2 receptor. Recent homologous binding studies, however, have shown that neurokinin A is also a high-affinity ligand for the tachykinin NK1 receptor. In this report, we demonstrate that a photoreactive neurokinin A analogue specifically labels the NK1 receptor in rat submandibular gland membranes and show via bioassay that neurokinin A is a potent stimulator of salivary secretion. Through the use of specific non-peptide antagonists in both photolabeling and functional assays, we unequivocally demonstrate that neurokinin A can specifically interact with the NK1 receptor in vivo and elicit NK1 receptor-mediated physiological responses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-147 |
| Number of pages | 5 |
| Journal | European Journal of Pharmacology |
| Volume | 423 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jul 6 2001 |
| Externally published | Yes |
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Keywords
- Bioassay
- Neurokinin A
- Photoaffinity labeling
- Tachykinin NK receptor
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Direct evidence for the interaction of neurokinin A with the tachykinin NK1 receptor in tissue. / Bremer, Andrew A.; Tansky, Morris F.; Wu, Meiye; Boyd, Norman D.; Leeman, Susan E.
In: European Journal of Pharmacology, Vol. 423, No. 2-3, 06.07.2001, p. 143-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct evidence for the interaction of neurokinin A with the tachykinin NK1 receptor in tissue
AU - Bremer, Andrew A.
AU - Tansky, Morris F.
AU - Wu, Meiye
AU - Boyd, Norman D.
AU - Leeman, Susan E.
PY - 2001/7/6
Y1 - 2001/7/6
N2 - Neurokinin A (NKA) is a tachykinin peptide that binds with high affinity to the tachykinin NK2 receptor. Recent homologous binding studies, however, have shown that neurokinin A is also a high-affinity ligand for the tachykinin NK1 receptor. In this report, we demonstrate that a photoreactive neurokinin A analogue specifically labels the NK1 receptor in rat submandibular gland membranes and show via bioassay that neurokinin A is a potent stimulator of salivary secretion. Through the use of specific non-peptide antagonists in both photolabeling and functional assays, we unequivocally demonstrate that neurokinin A can specifically interact with the NK1 receptor in vivo and elicit NK1 receptor-mediated physiological responses.
AB - Neurokinin A (NKA) is a tachykinin peptide that binds with high affinity to the tachykinin NK2 receptor. Recent homologous binding studies, however, have shown that neurokinin A is also a high-affinity ligand for the tachykinin NK1 receptor. In this report, we demonstrate that a photoreactive neurokinin A analogue specifically labels the NK1 receptor in rat submandibular gland membranes and show via bioassay that neurokinin A is a potent stimulator of salivary secretion. Through the use of specific non-peptide antagonists in both photolabeling and functional assays, we unequivocally demonstrate that neurokinin A can specifically interact with the NK1 receptor in vivo and elicit NK1 receptor-mediated physiological responses.
KW - Bioassay
KW - Neurokinin A
KW - Photoaffinity labeling
KW - Tachykinin NK receptor
UR - http://www.scopus.com/inward/record.url?scp=0035816414&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035816414&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(01)01107-4
DO - 10.1016/S0014-2999(01)01107-4
M3 - Article
C2 - 11448478
AN - SCOPUS:0035816414
VL - 423
SP - 143
EP - 147
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -