Direct electrochemistry of human, bovine and porcine cytochrome P450c17

D. L. Johnson, Alan J Conley, Lisa L. Martin

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The direct electrochemistry of human, bovine and porcine cytochrome P450c17 (CYP17) has been examined on an edge-oriented pyrolytic graphite electrode. The recombinant protein was immobilized on an electrode modified with a surfactant to simulate the environment of a biological membrane, and hence physiological electron-transfer conditions. The P450 enzymes all retained 'electron-transfer' activity while immobilized at the electrode surface as assessed by the presence of catalytic signals under aerobic conditions. The redox potentials for porcine P450c17 were more positive (anodic) than both the human and bovine forms, perhaps reflecting the differences in substrate specificity for these species. In addition, these enzymes were all influenced by pH, consistent with a single proton associated with the single electron-transfer event. Ionic strength of the buffer medium also shifted the redox potentials towards positive, suggesting that electrostatic forces contribute to the protein environment required for the electron-transfer process. The effect of substrate on the redox potential for each P450c17 was measured in the presence of pregnenolone, progesterone, 17α-hydroxypregnenolone and 17α-hydroxyprogesterone. However, no influence on the redox parameters was observed.

Original languageEnglish (US)
Pages (from-to)349-359
Number of pages11
JournalJournal of Molecular Endocrinology
Volume36
Issue number2
DOIs
StatePublished - Apr 2006

Fingerprint

Electrochemistry
Cytochromes
Oxidation-Reduction
Swine
Electrons
Electrodes
17-alpha-Hydroxypregnenolone
17-alpha-Hydroxyprogesterone
Pregnenolone
Graphite
Substrate Specificity
Static Electricity
Recombinant Proteins
Surface-Active Agents
Osmolar Concentration
Cytochrome P-450 Enzyme System
Progesterone
Protons
Buffers
Membranes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Direct electrochemistry of human, bovine and porcine cytochrome P450c17. / Johnson, D. L.; Conley, Alan J; Martin, Lisa L.

In: Journal of Molecular Endocrinology, Vol. 36, No. 2, 04.2006, p. 349-359.

Research output: Contribution to journalArticle

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