Direct dose confirmation of quantitative autoradiography with micro-TLD measurements for radioimmunotherapy

M. H. Griffith, E. D. Yorke, B. W. Wessels, Gerald L Denardo, W. P. Neacy

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Autoradiography has shown marked heterogeneous distribution of radioactivity in all ten radiolabeled monoclonal antibody/tumor combinations evaluated by our laboratories for radioimmunotherapy (RIT) in mice. Quantitative autoradiography was performed on two of these combinations (131I-B72.3/colorectal carcinoma and 131I-LYM-1/Raji B-cell lymphoma) to obtained a correlation of film density with radiolabeled antibody distribution. Through the use of sectioned mini-thermoluminescent dosimeter(s) (TLD) or micro-TLD, isodose curves were generated from the film gradient density lines. A computer program was written to compare theoretical absorbed dose calculations to measured micro-TLD values. First-order agreement was reached for both antibody/tumor systems: (a) B72.3/colorectal system-810 cGy measured/824 cGy calculated per 200 μCi injected and (b) LYM-1/lymphoma system-1,740 cGy measured/1,580 cGy calculated per 656 μCi injected (1 cGy = 1 rad). Additionally, the measured absorbed dose heterogeneity over a 500-μm length of up to 400% which suggests that the use of quantitative autoradiography is necessary in order to correctly determine the underlying radiobiological effects of RIT. Theoretical computer modeling based on similar autoradiographic activity distributions has also provided a convenient means of assessing absorbed dose variation patterns from other radiolabels such as 90Y.

Original languageEnglish (US)
Pages (from-to)1795-1809
Number of pages15
JournalJournal of Nuclear Medicine
Volume29
Issue number11
StatePublished - 1988
Externally publishedYes

Fingerprint

Radioimmunotherapy
Autoradiography
Neoplasm Antibodies
B-Cell Lymphoma
Radioactivity
Colorectal Neoplasms
Lymphoma
Software
Monoclonal Antibodies
Antibodies
Neoplasms
B72.3 antibody

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Direct dose confirmation of quantitative autoradiography with micro-TLD measurements for radioimmunotherapy. / Griffith, M. H.; Yorke, E. D.; Wessels, B. W.; Denardo, Gerald L; Neacy, W. P.

In: Journal of Nuclear Medicine, Vol. 29, No. 11, 1988, p. 1795-1809.

Research output: Contribution to journalArticle

Griffith, M. H. ; Yorke, E. D. ; Wessels, B. W. ; Denardo, Gerald L ; Neacy, W. P. / Direct dose confirmation of quantitative autoradiography with micro-TLD measurements for radioimmunotherapy. In: Journal of Nuclear Medicine. 1988 ; Vol. 29, No. 11. pp. 1795-1809.
@article{0f2d8451b32d4ca388379cd9a985a6d8,
title = "Direct dose confirmation of quantitative autoradiography with micro-TLD measurements for radioimmunotherapy",
abstract = "Autoradiography has shown marked heterogeneous distribution of radioactivity in all ten radiolabeled monoclonal antibody/tumor combinations evaluated by our laboratories for radioimmunotherapy (RIT) in mice. Quantitative autoradiography was performed on two of these combinations (131I-B72.3/colorectal carcinoma and 131I-LYM-1/Raji B-cell lymphoma) to obtained a correlation of film density with radiolabeled antibody distribution. Through the use of sectioned mini-thermoluminescent dosimeter(s) (TLD) or micro-TLD, isodose curves were generated from the film gradient density lines. A computer program was written to compare theoretical absorbed dose calculations to measured micro-TLD values. First-order agreement was reached for both antibody/tumor systems: (a) B72.3/colorectal system-810 cGy measured/824 cGy calculated per 200 μCi injected and (b) LYM-1/lymphoma system-1,740 cGy measured/1,580 cGy calculated per 656 μCi injected (1 cGy = 1 rad). Additionally, the measured absorbed dose heterogeneity over a 500-μm length of up to 400{\%} which suggests that the use of quantitative autoradiography is necessary in order to correctly determine the underlying radiobiological effects of RIT. Theoretical computer modeling based on similar autoradiographic activity distributions has also provided a convenient means of assessing absorbed dose variation patterns from other radiolabels such as 90Y.",
author = "Griffith, {M. H.} and Yorke, {E. D.} and Wessels, {B. W.} and Denardo, {Gerald L} and Neacy, {W. P.}",
year = "1988",
language = "English (US)",
volume = "29",
pages = "1795--1809",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
publisher = "Society of Nuclear Medicine Inc.",
number = "11",

}

TY - JOUR

T1 - Direct dose confirmation of quantitative autoradiography with micro-TLD measurements for radioimmunotherapy

AU - Griffith, M. H.

AU - Yorke, E. D.

AU - Wessels, B. W.

AU - Denardo, Gerald L

AU - Neacy, W. P.

PY - 1988

Y1 - 1988

N2 - Autoradiography has shown marked heterogeneous distribution of radioactivity in all ten radiolabeled monoclonal antibody/tumor combinations evaluated by our laboratories for radioimmunotherapy (RIT) in mice. Quantitative autoradiography was performed on two of these combinations (131I-B72.3/colorectal carcinoma and 131I-LYM-1/Raji B-cell lymphoma) to obtained a correlation of film density with radiolabeled antibody distribution. Through the use of sectioned mini-thermoluminescent dosimeter(s) (TLD) or micro-TLD, isodose curves were generated from the film gradient density lines. A computer program was written to compare theoretical absorbed dose calculations to measured micro-TLD values. First-order agreement was reached for both antibody/tumor systems: (a) B72.3/colorectal system-810 cGy measured/824 cGy calculated per 200 μCi injected and (b) LYM-1/lymphoma system-1,740 cGy measured/1,580 cGy calculated per 656 μCi injected (1 cGy = 1 rad). Additionally, the measured absorbed dose heterogeneity over a 500-μm length of up to 400% which suggests that the use of quantitative autoradiography is necessary in order to correctly determine the underlying radiobiological effects of RIT. Theoretical computer modeling based on similar autoradiographic activity distributions has also provided a convenient means of assessing absorbed dose variation patterns from other radiolabels such as 90Y.

AB - Autoradiography has shown marked heterogeneous distribution of radioactivity in all ten radiolabeled monoclonal antibody/tumor combinations evaluated by our laboratories for radioimmunotherapy (RIT) in mice. Quantitative autoradiography was performed on two of these combinations (131I-B72.3/colorectal carcinoma and 131I-LYM-1/Raji B-cell lymphoma) to obtained a correlation of film density with radiolabeled antibody distribution. Through the use of sectioned mini-thermoluminescent dosimeter(s) (TLD) or micro-TLD, isodose curves were generated from the film gradient density lines. A computer program was written to compare theoretical absorbed dose calculations to measured micro-TLD values. First-order agreement was reached for both antibody/tumor systems: (a) B72.3/colorectal system-810 cGy measured/824 cGy calculated per 200 μCi injected and (b) LYM-1/lymphoma system-1,740 cGy measured/1,580 cGy calculated per 656 μCi injected (1 cGy = 1 rad). Additionally, the measured absorbed dose heterogeneity over a 500-μm length of up to 400% which suggests that the use of quantitative autoradiography is necessary in order to correctly determine the underlying radiobiological effects of RIT. Theoretical computer modeling based on similar autoradiographic activity distributions has also provided a convenient means of assessing absorbed dose variation patterns from other radiolabels such as 90Y.

UR - http://www.scopus.com/inward/record.url?scp=0023781556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023781556&partnerID=8YFLogxK

M3 - Article

C2 - 3263479

AN - SCOPUS:0023781556

VL - 29

SP - 1795

EP - 1809

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

SN - 0161-5505

IS - 11

ER -