Abstract
Modern force microscopy techniques allow researchers to use mechanical forces to probe interactions between biomolecules. However, such measurements often happen in nonequilibrium regime, which precludes straightforward extraction of the equilibrium energy information. Here we use the work-averaging method based on Jarzynski equality to reconstruct the equilibrium interaction potential from the unbinding of a complementary 14-mer DNA duplex from the results of nonequilibrium single-molecule measurements. The reconstructed potential reproduces most of the features of the DNA stretching transition, previously observed only in equilibrium stretching of long DNA sequences. We also compare the reconstructed potential with the thermodynamic parameters of DNA duplex unbinding and show that the reconstruction accurately predicts duplex melting enthalpy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4792-4794 |
| Number of pages | 3 |
| Journal | Applied Physics Letters |
| Volume | 85 |
| Issue number | 20 |
| DOIs | |
| State | Published - Nov 15 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
Direct determination of the equilibrium unbinding potential profile for a short DNA duplex from force spectroscopy data. / Noy, Aleksandr.
In: Applied Physics Letters, Vol. 85, No. 20, 15.11.2004, p. 4792-4794.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct determination of the equilibrium unbinding potential profile for a short DNA duplex from force spectroscopy data
AU - Noy, Aleksandr
PY - 2004/11/15
Y1 - 2004/11/15
N2 - Modern force microscopy techniques allow researchers to use mechanical forces to probe interactions between biomolecules. However, such measurements often happen in nonequilibrium regime, which precludes straightforward extraction of the equilibrium energy information. Here we use the work-averaging method based on Jarzynski equality to reconstruct the equilibrium interaction potential from the unbinding of a complementary 14-mer DNA duplex from the results of nonequilibrium single-molecule measurements. The reconstructed potential reproduces most of the features of the DNA stretching transition, previously observed only in equilibrium stretching of long DNA sequences. We also compare the reconstructed potential with the thermodynamic parameters of DNA duplex unbinding and show that the reconstruction accurately predicts duplex melting enthalpy.
AB - Modern force microscopy techniques allow researchers to use mechanical forces to probe interactions between biomolecules. However, such measurements often happen in nonequilibrium regime, which precludes straightforward extraction of the equilibrium energy information. Here we use the work-averaging method based on Jarzynski equality to reconstruct the equilibrium interaction potential from the unbinding of a complementary 14-mer DNA duplex from the results of nonequilibrium single-molecule measurements. The reconstructed potential reproduces most of the features of the DNA stretching transition, previously observed only in equilibrium stretching of long DNA sequences. We also compare the reconstructed potential with the thermodynamic parameters of DNA duplex unbinding and show that the reconstruction accurately predicts duplex melting enthalpy.
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U2 - 10.1063/1.1819982
DO - 10.1063/1.1819982
M3 - Article
VL - 85
SP - 4792
EP - 4794
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 20
ER -